User:University of Washington/1 July 2008

From 2008.igem.org

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== LuxR from AraC and TetR ==
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- AraC sequences matched the biobrick (BBa_R0080).
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== LuxR from pLAc ==
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- Miniprep done on R0010 and J04430 transformed e.coli. DNA was submitted for sequencing of the parts.
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- Part I0462 was again transformed into a different e.coli strain.
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== BioBrick Promoter Measurements ==
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- All overnight cultures showed successful transformation by growing on plates with kanamycin.
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- Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours.
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- 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius.
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- The remaining cultures were placed back into the 37 degree incubator to grow overnight.
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== Conjugation ==
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·Transform RP4 into S17-1 cells

Latest revision as of 23:03, 2 July 2008

Contents

LuxR from AraC and TetR

- AraC sequences matched the biobrick (BBa_R0080).

LuxR from pLAc

- Miniprep done on R0010 and J04430 transformed e.coli. DNA was submitted for sequencing of the parts.

- Part I0462 was again transformed into a different e.coli strain.


BioBrick Promoter Measurements

- All overnight cultures showed successful transformation by growing on plates with kanamycin.

- Single colonies were added to 5 mL of TSY media, then incubated at 37 degrees Celsius in a rotator for 5 hours.

- 750 uL of the incubated cells were added to 750 uL of 40% glycerol, incubated at room temperature for 30 minutes, then stored at -80 degrees Celsius.

- The remaining cultures were placed back into the 37 degree incubator to grow overnight.

Conjugation

·Transform RP4 into S17-1 cells


Team:University_of_Washington/Notebook#Notebook

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