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Team:NYMU-Taipei/Project/Phosphate/Simulations/Phosphate
From 2008.igem.org
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* [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6998957 Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli, JB 1980 (G R Willsky and M H Malamy)] | * [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6998957 Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli, JB 1980 (G R Willsky and M H Malamy)] | ||
| - | * high affinity-low velocity Pst (phosphate specific transport) system have a Km of 0.43 +/- 0.2 microM Pi and a Vmax of 15.9 +/- 0.3 nmol of Pi | + | * high affinity-low velocity Pst (phosphate specific transport) system have a Km of 0.43 +/- 0.2 microM Pi and a Vmax of 15.9 +/- 0.3 nmol of Pi (mg [dry weight]<sup>-1</sup>min<sup>-1</sup>) |
| - | * low affinity-high velocity Pit (Pi transport) system have a Km of 38.2 +/- 0.4 microM and a Vmax of 55 +/- 1.9 nmol of Pi | + | * low affinity-high velocity Pit (Pi transport) system have a Km of 38.2 +/- 0.4 microM and a Vmax of 55 +/- 1.9 nmol of Pi (mg [dry weight]<sup>-1</sup>min<sup>-1</sup>) |
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| - | == Phosphate Concentrations == | + | == Phosphate Concentrations of normal and CKD patient == |
* Normal adult (0.81 to 1.45 mmol/L) | * Normal adult (0.81 to 1.45 mmol/L) | ||
| - | * Chronic Kidney Disease patient (1.29 to 2.096 mmol/L) | + | * Chronic Kidney Disease (CDK) patient (1.29 to 2.096 mmol/L) |
* [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck] | * [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck] | ||
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| + | == Simulation of Phosphate Absorption == | ||
| + | * assume 1g/L (dry weight) therapeutic bacteria in the human intestine | ||
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| + | {| | ||
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| + | [[Image:PST 2000nmol pi.jpg|400px]] | ||
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| + | * PST system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 2 hours | ||
| + | |- | ||
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| + | [[Image:PIT 2000nmol pi.jpg.jpg|400px]] | ||
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| + | * PIT system alone can reduce the environmental phosphate from 2000nmol to 100nmol within 1 hours | ||
| + | |} | ||
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| + | * To decrease the illness caused by fast phosphate removal, we prefer to use PST system | ||
Latest revision as of 09:16, 30 October 2008
Transportation Efficiency of PIT and PST system
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Phosphate Concentrations of normal and CKD patient
- Normal adult (0.81 to 1.45 mmol/L)
- Chronic Kidney Disease (CDK) patient (1.29 to 2.096 mmol/L)
- [http://www.merck.com/mmpe/sec12/ch156/ch156h.html Disorders of Phosphate Concentration by merck]
Simulation of Phosphate Absorption
- assume 1g/L (dry weight) therapeutic bacteria in the human intestine
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- To decrease the illness caused by fast phosphate removal, we prefer to use PST system
