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Template:Team:CityColSanFrancisco/Notebook/Notebook/DV notes
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Hello <br> | Hello <br> | ||
6/29/2009 | 6/29/2009 | ||
| - | R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species. | + | R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect. |
| + | Plan now to: | ||
| + | - inoculate liquid media with single colonies from these strains. | ||
| + | -Inoculate | ||
| + | - Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis) | ||
| + | |||
| + | Tomorrow: | ||
| + | -Order designed primers | ||
| + | -Grow mutants in liquid media<br> | ||
| + | |||
| + | 6/30/2009 <br> | ||
| + | Grew mutants alongside WT Shewanella. They don't look alike. I'm suspicious. | ||
Latest revision as of 17:37, 30 June 2009
Hello
6/29/2009
R. palustris TIE-1 and S. oneidensis WT strains appear to have grown. Gram staining of S. oneidensis WT revealed what appeared to be gram-negative rods; primers being designed for a unique S. oneidensis sequence to confirm species is what we suspect.
Plan now to:
- inoculate liquid media with single colonies from these strains.
-Inoculate
- Re-streak individual colonies from these strains, along with TIE-3 (R. palustris) and MtrB and OmpB strains (S. oneidensis)
Tomorrow:
-Order designed primers
-Grow mutants in liquid media
6/30/2009
Grew mutants alongside WT Shewanella. They don't look alike. I'm suspicious.
