"
Minnesota/25 June 2008
From 2008.igem.org
(Difference between revisions)
Emartin9808 (Talk | contribs) |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{|style="align:left" width="965" | {|style="align:left" width="965" | ||
|- | |- | ||
| - | |'''[[Team:Minnesota/NotebookComparator| Back to Notebook]]''' | + | |'''[[Team:Minnesota/NotebookComparator| Back to Notebook Home]]''' |
|- | |- | ||
|'''[[Minnesota/24 June 2008|Go to Previous Day (June 24)]]'''|| width=158|'''[[Minnesota/26 June 2008|Go to Next Day (June 26)]]''' | |'''[[Minnesota/24 June 2008|Go to Previous Day (June 24)]]'''|| width=158|'''[[Minnesota/26 June 2008|Go to Next Day (June 26)]]''' | ||
| Line 7: | Line 7: | ||
{| | {| | ||
|- | |- | ||
| - | |1. ''' | + | |1. '''Streak LB + antibiotic plate:''' |
|- | |- | ||
|a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours. | |a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours. | ||
|- | |- | ||
|b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow. | |b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow. | ||
| + | |- | ||
| + | |||
|- | |- | ||
|2. '''Begin 2mL glycerol stock culture:''' | |2. '''Begin 2mL glycerol stock culture:''' | ||
| Line 20: | Line 22: | ||
|- | |- | ||
|3. '''Transformation results''' | |3. '''Transformation results''' | ||
| + | |- | ||
| + | [[Image:DSCN2096.JPG|300px||center|thumb|Transformation reults]] | ||
|- | |- | ||
|4. '''2mL cultures for plasmid miniprep''' | |4. '''2mL cultures for plasmid miniprep''' | ||
|} | |} | ||
Latest revision as of 19:16, 1 July 2008
| Back to Notebook Home | |
| Go to Previous Day (June 24) | Go to Next Day (June 26) |
| 1. Streak LB + antibiotic plate: |
| a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours. |
| b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow. |
| 2. Begin 2mL glycerol stock culture: |
| a. The same colonies that were picked for the initial plasmid prep and the streaked plates directly above were again picked with a sterile pipette tip, and the tip was placed in 2mL LB broth + antibiotic (1uL/mL). All tubes were flamed immediately after opening and before closing and work was done near a flame to prevent contamination. |
| b. These were grown for approximately 18 hours @ 37C with shaking @ 220 rpm's. |
| 3. Transformation results |
| 4. 2mL cultures for plasmid miniprep |
