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Minnesota/25 June 2008
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| - | |1. ''' | + | |1. '''Streak LB + antibiotic plate:''' |
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|a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours. | |a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours. | ||
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|b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow. | |b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow. | ||
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|2. '''Begin 2mL glycerol stock culture:''' | |2. '''Begin 2mL glycerol stock culture:''' | ||
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|3. '''Transformation results''' | |3. '''Transformation results''' | ||
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| + | [[Image:DSCN2096.JPG|300px||center|thumb|Transformation reults]] | ||
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|4. '''2mL cultures for plasmid miniprep''' | |4. '''2mL cultures for plasmid miniprep''' | ||
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Latest revision as of 19:16, 1 July 2008
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| 1. Streak LB + antibiotic plate: |
| a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours. |
| b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow. |
| 2. Begin 2mL glycerol stock culture: |
| a. The same colonies that were picked for the initial plasmid prep and the streaked plates directly above were again picked with a sterile pipette tip, and the tip was placed in 2mL LB broth + antibiotic (1uL/mL). All tubes were flamed immediately after opening and before closing and work was done near a flame to prevent contamination. |
| b. These were grown for approximately 18 hours @ 37C with shaking @ 220 rpm's. |
| 3. Transformation results |
| 4. 2mL cultures for plasmid miniprep |
