Template:Team:UC Berkeley/Notebook/CC construction

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==K112500==
==K112500==
<pre>
<pre>
-
Construction of {<HA>} basic part K112501
+
Construction of {<HA>} basic part K112500
Wobble PCR of cc001/cc002                  ( 50 bp, EcoRI/BamHI)
Wobble PCR of cc001/cc002                  ( 50 bp, EcoRI/BamHI)
Line 18: Line 18:
==K112501==
==K112501==
<pre>
<pre>
-
Construction of {<HA!} basic part K112502
+
Construction of {<HA!} basic part K112501
Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
Line 33: Line 33:
==K112502==
==K112502==
<pre>
<pre>
-
Construction of {<myc>} basic part K112503
+
Construction of {<myc>} basic part K112502
Wobble PCR of cc004/cc005                  ( 51 bp, EcoRI/BamHI)
Wobble PCR of cc004/cc005                  ( 51 bp, EcoRI/BamHI)
Line 63: Line 63:
Construction of {<barnase>} basic part K112504
Construction of {<barnase>} basic part K112504
-
PCR CC007/CC008 on barnase gene            ( 351 bp, EcoRI/BamHI)
+
PCR CC007/CC008 on barnase gene            ( 361 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
Product is pBca1256-K112504
Line 70: Line 70:
CGATAgaattcATGaGATCTGCACAGG
CGATAgaattcATGaGATCTGCACAGG
CC008            Reverse Biobricking of {<barnase>}
CC008            Reverse Biobricking of {<barnase>}
-
CCAGTggatcCTCTGATTTTTGTAAAGGTC
+
CCAGTggatccTCTGATTTTTGTAAAGGTCTG
</pre>
</pre>
==K112505==
==K112505==
<pre>
<pre>
-
Construction of {barnase>} basic part K112505
+
Construction of {<barnase!} basic part K112505
-
PCR CC009/CC008 on barnase gene            ( 354 bp, EcoRI/BamHI)
+
PCR CC007/CC009 on barnase gene            ( 364 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112505
Product is pBca1256-K112505
--------------------------------------------
--------------------------------------------
-
cc009           Forward Biobricking of {barnase>}
+
cc007           Forward Biobricking of {<barnase!}  
-
CGATAgaattcATGagatctATGGCACAGGTTATCAACACGTTTG
+
CGATAgaattcATGaGATCTGCACAGG
-
cc008           Reverse Biobricking of {barnase>}
+
CC009           Reverse Biobricking of {<barnase!}  
-
CCAGTggatcCTCTGATTTTTG
+
CCAGTggatcCTTATCTGATTTTTG
</pre>
</pre>
-
==K112506==
+
==K112232 (Molly's)==
<pre>
<pre>
-
Construction of {<barnase!} basic part K112506
+
Construction of {rbs.barstar!} Biobrick Part K112232
 +
PCR mea035/mea034 on Bacillus amyloliquefaciens gen          (320 bp, EcoRI/BamHI)
 +
Sub into pBca1256                                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112232
 +
----------------------------------------
 +
mea035          Forward Biobricking of {rbs.barstar!} 
 +
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
 +
mea034          Reverse Biobricking of {rbs.barstar!} 
 +
cgttaGGATCCttaagaaagtatgatggtgatgtcgcagcc
 +
</pre>
 +
 
 +
 
 +
== K112233 (Molly's) ==
 +
<pre>
 +
Construction of {<phoA>} Biobrick Part K112233
 +
PCR mea036/mea037 on pBca9145-Bca1126 (1219 bp, EcoRI/BamHI)
 +
Sub into pBjh1601AC-Bca1144 (EcoRI/BamHI, 3195,910 L)
 +
Product is pBjh1601AC-K112233
 +
----------------------------------------
 +
mea036  Forward Biobricking of {<phoA>} cgatagaattcatgAGATCTGGAGATTCTG
 +
mea037  Reverse Biobricking of {<phoA>} cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG
 +
</pre>
 +
 
 +
 
 +
== K112506 ==
 +
 
 +
<pre>
 +
Construction of {<HA>} basic part K112506
-
PCR CC007/CC010 on barnase gene            ( 354 bp, EcoRI/BamHI)
+
Wobble PCR of cc001/cc010                  ( 51 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112506
Product is pBca1256-K112506
--------------------------------------------
--------------------------------------------
-
cc007            Forward Biobricking of {<barnase!}  
+
cc001            Forward Biobricking of {<HA>}  
-
CGATAgaattcATGaGATCTGCACA
+
CgATAgaattcATGagatcttacccatacgacgtcccagac
-
CC010            Reverse Biobricking of {<barnase!}  
+
 
-
CCAGTggatcCTTATCTGATTTTTG
+
cc010            Reverse biobricking of {<HA>}
 +
ccagtggatcccccagcgtagtctgggacgtcgtatggg
</pre>
</pre>
-
==K112507==
+
 
 +
== K112507 ==
 +
 
<pre>
<pre>
-
Construction of {<barnase!} basic part K112506
+
Construction of {<HA!} basic part K112507
-
PCR mea035/mea034 on Bacillus amyloliquefaciens gen.  (320 bp, EcoRI/BamHI)
+
Wobble PCR of cc001/cc011                  ( 54 bp, EcoRI/BamHI)
-
Sub into pBca1256                                     (EcoRI/BamHI, 2472+910, L)
+
Sub into pBca1256                         (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112507
Product is pBca1256-K112507
-
----------------------------------------
+
--------------------------------------------
-
mea035      Forward Biobricking of {rbs.barstar!}
+
cc001            Forward Biobricking of {<HA>}  
-
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
+
CgATAgaattcATGagatcttacccatacgacgtcccagac
-
mea034      Reverse Biobricking of {rbs.barstar!}
+
 
-
cgttaGGATCCttaagaaagtatgatggtgatgtcgc
+
cc011            Reverse biobricking of {<HA!}
 +
ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag
 +
 
</pre>
</pre>
 +
 +
 +
--------------------------------------------------------------------------------------------------------------
 +
 +
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Cici_Chen|Cici Chen]] </div>
 +
<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook| All notebook]] </div>

Latest revision as of 01:58, 25 July 2008

Contents


K112500

Construction of {<HA>} basic part K112500

Wobble PCR of cc001/cc002                  ( 50 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112500
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc002             Reverse biobricking of {<HA>} 
ccagtggatccccagcgtagtctgggacgtcgtatggg

K112501

Construction of {<HA!} basic part K112501

Wobble PCR of cc001/cc003                  ( 53 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112501
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc003             Reverse biobricking of {<HA!}
ccagtggatccttaccagcgtagtctgggacgtcgtatgggtaag

K112502

Construction of {<myc>} basic part K112502

Wobble PCR of cc004/cc005                  ( 51 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112502
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc005             Reverse Biobricking of {<myc>}
ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG

K112503

Construction of {<myc!} basic part K112503

Wobble PCR of cc004/cc006                  ( 54 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI,2227+ 910,L)
Product is pBca1256-K112503
--------------------------------------------
cc004             Forward Biobricking of {<myc>}
CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG
cc006             Reverse Biobricking of {<myc!}
ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC

K112504

Construction of {<barnase>} basic part K112504

PCR CC007/CC008 on barnase gene            ( 361 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112504
--------------------------------------------
cc007            Forward Biobricking of {<barnase>}
CGATAgaattcATGaGATCTGCACAGG
CC008            Reverse Biobricking of {<barnase>}
CCAGTggatccTCTGATTTTTGTAAAGGTCTG

K112505

Construction of {<barnase!} basic part K112505

PCR CC007/CC009 on barnase gene            ( 364 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112505
--------------------------------------------
cc007            Forward Biobricking of {<barnase!} 
CGATAgaattcATGaGATCTGCACAGG
CC009            Reverse Biobricking of {<barnase!} 
CCAGTggatcCTTATCTGATTTTTG

K112232 (Molly's)

Construction of {rbs.barstar!} Biobrick Part K112232
PCR mea035/mea034 on Bacillus amyloliquefaciens gen          (320 bp, EcoRI/BamHI)
Sub into pBca1256                                            (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112232
----------------------------------------
mea035           Forward Biobricking of {rbs.barstar!}  
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg
mea034           Reverse Biobricking of {rbs.barstar!}  
cgttaGGATCCttaagaaagtatgatggtgatgtcgcagcc


K112233 (Molly's) 

Construction of {<phoA>} Biobrick Part K112233
PCR mea036/mea037 on pBca9145-Bca1126		(1219 bp, EcoRI/BamHI)
Sub into pBjh1601AC-Bca1144			(EcoRI/BamHI, 3195,910 L)
Product is pBjh1601AC-K112233
----------------------------------------
mea036   Forward Biobricking of {<phoA>}	cgatagaattcatgAGATCTGGAGATTCTG
mea037   Reverse Biobricking of {<phoA>}	cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG


K112506 

Construction of {<HA>} basic part K112506

Wobble PCR of cc001/cc010                  ( 51 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112506
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc010             Reverse biobricking of {<HA>}
ccagtggatcccccagcgtagtctgggacgtcgtatggg


K112507 

Construction of {<HA!} basic part K112507

Wobble PCR of cc001/cc011                  ( 54 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112507
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc011             Reverse biobricking of {<HA!}
ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag


Cici Chen
All notebook
Retrieved from "http://2008.igem.org/Template:Team:UC_Berkeley/Notebook/CC_construction"