Team:KULeuven/Tools/PCR

From 2008.igem.org

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{| align="center" width=80%
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! colspan="3" style="background:#003E81; color:white" |PCR:
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! colspan="3" style="background:#003E81; color:white" |'''PCR:'''
|-valign="top"
|-valign="top"
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|
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| width=20% |
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{| cellspacing="1"
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{| cellspacing="1" width=100%
|style="background:#003E81; color:white"|PCR Settings
|style="background:#003E81; color:white"|PCR Settings
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|-bgcolor="#66FFCC"
 
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|Annealing (°C)
 
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
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|{{{1}}}°
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| width="200" |Annealing (°C)
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|-bgcolor="#66FFCC"
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|-bgcolor="#f0f0f0"
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|{{{tanneal}}}°
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|-bgcolor="#99CCFF"
|Time Elongation
|Time Elongation
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|-bgcolor="#f0f0f0"
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|{{{telong}}}
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
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|{{{2}}}
 
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|-bgcolor="#66FFCC"
 
|Number of Cycles
|Number of Cycles
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|-bgcolor="#99CCFF"
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|-bgcolor="#f0f0f0"
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|x {{{3}}}
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|{{{ncycles}}}
|}
|}
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|
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| width=60% |
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{| cellspacing="1"
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{| cellspacing="1" width=100%
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|bgcolor="#66FFCC"|Buffer (5x)
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| width="200" bgcolor="#99CCFF"|Buffer (5x)
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|bgcolor="#99CCFF" colspan="2"|µL x
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|bgcolor="#f0f0f0" colspan="2"|{{{buffer}}}
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|-
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|bgcolor="#66FFCC"|MgCl<sub>2</sub> 10 µM
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|bgcolor="#99CCFF"|MgCl<sub>2</sub> 10 µM
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|bgcolor="#99CCFF" colspan="2"|[]
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|bgcolor="#f0f0f0" colspan="2"|{{{mgcl}}}
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|-
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|bgcolor="#66FFCC"|dNTP 10 µM
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|bgcolor="#99CCFF"|dNTP 10 µM
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|bgcolor="#99CCFF" colspan="2"|[]
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|bgcolor="#f0f0f0" colspan="2"|{{{dntp}}}
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|-
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|bgcolor="#66FFCC"|Oligo F 10 µM
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|bgcolor="#99CCFF"|Oligo F 10 µM
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|bgcolor="#99CCFF"|(Number of the Fw Primer)
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|bgcolor="#f0f0f0"|{{{fwprimername}}}
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|bgcolor="#99CCFF"|µL
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|bgcolor="#f0f0f0"| {{{fwprimerconc}}}
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|-
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|bgcolor="#66FFCC"|Oligo R 10 µM
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|bgcolor="#99CCFF"|Oligo R 10 µM
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|bgcolor="#99CCFF"|(Number of the Rv Primer)
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|bgcolor="#f0f0f0"|{{{rvprimername}}}
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|bgcolor="#99CCFF"|µL
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|bgcolor="#f0f0f0"|{{{rvprimerconc}}}
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|-
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|bgcolor="#66FFCC"|Water
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|bgcolor="#99CCFF"|Water
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|bgcolor="#99CCFF" colspan="2"|µL
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|bgcolor="#f0f0f0" colspan="2"|{{{water}}}
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|-
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|bgcolor="#66FFCC"|Polymerase
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|bgcolor="#99CCFF"|Polymerase
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|bgcolor="#99CCFF" colspan="2"|
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|bgcolor="#f0f0f0" colspan="2"|{{{polymerase}}}
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|-
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|bgcolor="#66FFCC"|DNA
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|bgcolor="#99CCFF"|DNA
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|bgcolor="#99CCFF" colspan="2"|
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|bgcolor="#f0f0f0" colspan="2"|{{{dna}}}
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|}
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|
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| width=20% |
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{| cellspacing="1"
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{| cellspacing="1" width=100%
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|bgcolor="#66FFCC"|Expected Size
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| width="200" bgcolor="#99CCFF"|Expected Size
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|-
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|bgcolor="#99CCFF"|{{{size}}}
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|bgcolor="#f0f0f0"|{{{size}}}
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|-
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|bgcolor="#66FFCC"|Success
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|bgcolor="#99CCFF"|Success
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|-
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|bgcolor="#99CCFF"|{{{success}}}
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|bgcolor="#f0f0f0"|{{{success}}}
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|-
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|bgcolor="#66FFCC"|Image (click to enlarge)
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|bgcolor="#99CCFF"|Image (click to enlarge)
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|-
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|bgcolor="#99CCFF"|[[Image:{{{Image}}}|30px]]
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|bgcolor="#f0f0f0"|[[Image:{{{image}}}|30px]]
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|-
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|bgcolor="#66FFCC"|Band (0=ladder)
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|bgcolor="#99CCFF"|Lane (0=ladder)
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|-
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|bgcolor="#99CCFF"|{{{band}}}
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|bgcolor="#f0f0f0"|{{{lane}}}
|}
|}
|}
|}

Latest revision as of 22:53, 18 July 2008

PCR:
PCR Settings
Annealing (°C)
{{{tanneal}}}°
Time Elongation
{{{telong}}}
Number of Cycles
{{{ncycles}}}
Buffer (5x) {{{buffer}}}
MgCl2 10 µM {{{mgcl}}}
dNTP 10 µM {{{dntp}}}
Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}}
Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}}
Water {{{water}}}
Polymerase {{{polymerase}}}
DNA {{{dna}}}
Expected Size
{{{size}}}
Success
{{{success}}}
Image (click to enlarge)
[[Image:{{{image}}}|30px]]
Lane (0=ladder)
{{{lane}}}
Retrieved from "http://2008.igem.org/Team:KULeuven/Tools/PCR"