Team:NTU-Singapore/Notebook/3 July 2008
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(New page: Chin Chong & Zhen Fu *Carried out characterization of Standard, Low, Medium and High Promoters that were sent to us in the Newsletter Vol. 1 #Cells that were grown overnight were diluted a...) |
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+ | =Thursday 3 July= | ||
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+ | ==Chin Chong & Zhen Fu== | ||
*Carried out characterization of Standard, Low, Medium and High Promoters that were sent to us in the Newsletter Vol. 1 | *Carried out characterization of Standard, Low, Medium and High Promoters that were sent to us in the Newsletter Vol. 1 | ||
#Cells that were grown overnight were diluted and grown to OD 1.2 | #Cells that were grown overnight were diluted and grown to OD 1.2 | ||
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**Cells fluorescene intensity was directly linked to the strenght of the promoters. The stronger the promoters, the brighter the fluorescene appears to be | **Cells fluorescene intensity was directly linked to the strenght of the promoters. The stronger the promoters, the brighter the fluorescene appears to be | ||
**Pictures and some movie clips were taken | **Pictures and some movie clips were taken | ||
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+ | *E7+Imm successfully PCRed | ||
+ | **Optimizing PCR reaction | ||
+ | ***Due to Self annealing primers(Primers-dimer), Increase Tm value and using 1ul of primers each instead of 2ul | ||
+ | *Staining of gel | ||
+ | **Unsuccessful initially with gel-star stain, however after post soaking of gel in stain buffer for 4hrs, bands became clearer and and missing bands reappeared. | ||
+ | ***New protocol for gel Runs--Post soak of gel in buffer for at least 30 mins | ||
+ | ==Choon Kit, Hung, Lu Chao:== | ||
+ | *Ligation of LacI-RBS34 and Fe-GFP again. | ||
+ | </div> |
Latest revision as of 02:24, 28 October 2008
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Thursday 3 July
Chin Chong & Zhen Fu
Choon Kit, Hung, Lu Chao:
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