Team:University of Ottawa/2 July 2008

From 2008.igem.org

(Difference between revisions)
(→Today in the Lab)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
 +
{|######Navigational Bar#######
 +
|}
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<script src="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBar.js" type="text/javascript"></script>
 +
<link href="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarHorizontal.css" rel="stylesheet" type="text/css" />
 +
</head>
 +
 +
<body>
 +
 +
<ul id="MenuBar1" class="MenuBarHorizontal">
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa">Home</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
 +
        </ul>
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Who_we_are">Who We Are</a>
 +
            <ul>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Advisors">Advisors</a></li>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Undergrads">Undergrads</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#What_we_did">What We've Done</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Where_we.27re_from">Where We're From</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Contact_Us">Contact Us</a></li>
 +
       
 +
      </ul>
 +
  </li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
 +
      <ul>
 +
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li>
 +
      </ul>
 +
  </li>
 +
  <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
 +
    <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li>
 +
    </ul>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li>   
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li>       
 +
      </ul>
 +
  </li>
 +
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
<script type="text/javascript">
 +
<!--
 +
var MenuBar1 = new Spry.Widget.MenuBar("MenuBar1", {imgDown:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarDownHover.gif", imgRight:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarRightHover.gif"});
 +
//-->
 +
</script>
 +
</body>
 +
</html>
 +
__TOC__
__TOC__
==Today in the Lab==
==Today in the Lab==
Line 7: Line 75:
::<li> We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning.
::<li> We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning.
:'''Transformation'''
:'''Transformation'''
-
::<li> The competent cells were transformed with the ligation product and left overnight.The receptor component Atcre was also integrated into competent cells.
+
::<li> The competent cells were transformed with the ligation product of all three samples and left overnight.The receptor component Atcre was also integrated into competent cells.
:'''Dehydrogenase component'''
:'''Dehydrogenase component'''
::<li> The dehydrogenase was received in the mail, I streaked the Ecoli containing the component to grow overnight in the incubator. Tomorrow we will inoculate for mini prep the following day.
::<li> The dehydrogenase was received in the mail, I streaked the Ecoli containing the component to grow overnight in the incubator. Tomorrow we will inoculate for mini prep the following day.
 +
'''Dan'''
 +
:'''PCR of pSSRE constucts'''
 +
::<li> Turned out to be a disaster. Had nonspecific binding and >4 bands. We would be better of going back to the original gel extraction products.
 +
:'''Digestion'''
 +
::<li> Now I am using the original gel extraction products (1,2,3,4) and

Latest revision as of 23:41, 28 October 2008

Untitled Document

 

 

Contents

Today in the Lab

Matt

Ligation
  • I am using three different samples of 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42.
  • A gel confirmation was run however nothing appeared on the gel due to a very low DNA concentration of the PTP2.
  • We decided it was better just to try and integrate into competent cells and incubate overnight for tomorrow morning.
    • Transformation
  • The competent cells were transformed with the ligation product of all three samples and left overnight.The receptor component Atcre was also integrated into competent cells.
    • Dehydrogenase component
  • The dehydrogenase was received in the mail, I streaked the Ecoli containing the component to grow overnight in the incubator. Tomorrow we will inoculate for mini prep the following day.

    • Dan

    PCR of pSSRE constucts
  • Turned out to be a disaster. Had nonspecific binding and >4 bands. We would be better of going back to the original gel extraction products.
    • Digestion
  • Now I am using the original gel extraction products (1,2,3,4) and
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/2_July_2008"