Team:University of Ottawa/3 July 2008
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(New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Competent Cells''' ::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of g...) |
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__TOC__ | __TOC__ | ||
==Today in the Lab== | ==Today in the Lab== | ||
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:'''Competent Cells''' | :'''Competent Cells''' | ||
::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth | ::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth | ||
+ | ::<li> The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow. | ||
+ | ::<li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli. | ||
:'''Ligation''' | :'''Ligation''' | ||
- | ::<li> Atcre was ligated to circularize the plasmid | + | ::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today. |
+ | '''Dan''' | ||
+ | :'''Atcre integration''' | ||
+ | ::<li> Plate had ~4 colonies so I decided to redo integration. | ||
+ | :'''1,2,3,4 and S,D,T ligation''' | ||
+ | ::<li> The concentration of these constructs was determined to be decent | ||
+ | ::<li> A gel was run to verify the integrity of the constructs, S,D,T showed some contamination | ||
+ | ::<li> 1,2,3,4 did not show up on the gel for unknown reasons. | ||
+ | |||
+ | '''Dan and Matt''' | ||
+ | :'''Integration''' | ||
+ | ::<li> Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated |
Latest revision as of 23:42, 28 October 2008
Contents |
Today in the Lab
Matt
- Competent Cells
- Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
- The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
- The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
- Ligation
- Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
Dan
- Atcre integration
- Plate had ~4 colonies so I decided to redo integration.
- 1,2,3,4 and S,D,T ligation
- The concentration of these constructs was determined to be decent
- A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
- 1,2,3,4 did not show up on the gel for unknown reasons.
Dan and Matt
- Integration
- Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated