Team:University of Ottawa/3 July 2008

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__TOC__
__TOC__
==Today in the Lab==
==Today in the Lab==
Line 4: Line 72:
:'''Competent Cells'''
:'''Competent Cells'''
::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
 +
::<li> The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
 +
::<li> The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
:'''Ligation'''
:'''Ligation'''
-
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again.
+
::<li> Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.
 +
'''Dan'''
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:'''Atcre integration'''
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::<li> Plate had ~4 colonies so I decided to redo integration.
 +
:'''1,2,3,4 and S,D,T ligation'''
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::<li> The concentration of these constructs was determined to be decent
 +
::<li> A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
 +
::<li> 1,2,3,4 did not show up on the gel for unknown reasons.
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'''Dan and Matt'''
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:'''Integration'''
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::<li> Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated

Latest revision as of 23:42, 28 October 2008

Untitled Document

 

 

Contents

Today in the Lab

Matt

Competent Cells
  • Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of growth
  • The cells containing the ligation product will then be inoculated and left to incubate overnight for a miniprep tomorrow.
  • The Ecoli containing the dehydrogenase component failed to grow from streaking a plate yesterday,I will try and streak again today and then glycerol stock the remaining Ecoli.
    • Ligation
  • Atcre was ligated to circularize the plasmid and will be integrated into Ecoli again today.

    • Dan

    Atcre integration
  • Plate had ~4 colonies so I decided to redo integration.
    • 1,2,3,4 and S,D,T ligation
  • The concentration of these constructs was determined to be decent
  • A gel was run to verify the integrity of the constructs, S,D,T showed some contamination
  • 1,2,3,4 did not show up on the gel for unknown reasons.

    • Dan and Matt

    Integration
  • Atcre, and 1S 1D 1T 2S 2D 2T 3S 3D 3T 4S 4D 4T were integrated
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/3_July_2008"