Team:NTU-Singapore/Project/Specification

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(The FLx800™ Fluorescence Microplate Reader)
 
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=Specification=
=Specification=
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==Introduction==
 
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The group proposed to discuss and investigate the working range of the parts and devices of our system
 
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*The proposed variables to investigate are: <br> i) Concentration of activator <br> ii) Time <br> iii) Temperature
 
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==Characterization of Parts==
 
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We are interested in characterizing the parts
 
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i) Lactose/IPTG sensitive Promoter pLac [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]
 
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ii) Iron Sensitive Promoter pFe [http://partsregistry.org/wiki/index.php?title=Part:BBa_I716014 BBa_I716014]
 
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The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: [http://partsregistry.org/Part:BBa_E0240 BBa_E02040]
 
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*For the pLac BBa_R0010, it is already available in the form we desired in the registry as [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]
 
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==The FLx800™ Fluorescence Microplate Reader==
 
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[[image: Ntu@iGEM_FLx_800_pic.jpg]]
 
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The FLx800™ Fluorescence Microplate Reader was available for our use courtesy of Bio-engineering division's lab.
 
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It was purchased from BioTek, for more information about the reader, you can refer to [http://www.biotek.com/products/product_detail.php?pid=117 The FLx800™ Fluorescence Microplate Reader]
 

Latest revision as of 06:15, 17 August 2008