Team:NTU-Singapore/Project/Specification

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Latest revision as of 06:15, 17 August 2008

Introduction
Specification
Construct design
Modelling&Implementation
Testing&Results
Conclusions

=Specification=

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 =Specification=
-==Introduction==
-The group proposed to discuss and investigate the working range of the parts and devices of our system
-*The proposed variables to investigate are: <br> i) Concentration of activator <br> ii) Time <br> iii) Temperature
-==Characterization of Parts==
-We are interested in characterizing the parts
-i) Lactose/IPTG sensitive Promoter pLac [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]
-ii) Iron Sensitive Promoter pFe [http://partsregistry.org/wiki/index.php?title=Part:BBa_I716014 BBa_I716014]
-The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: [http://partsregistry.org/Part:BBa_E0240 BBa_E02040]
-*For the pLac BBa_R0010, it is already available in the form we desired in the registry as [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]
-==The FLx800™ Fluorescence Microplate Reader==
-[[image: Ntu@iGEM_FLx_800_pic.jpg]]
-The FLx800™ Fluorescence Microplate Reader was available for our use courtesy of Division of Bio-engineering lab. It was purchased from BioTek and for more information about the reader, you can refer to [http://www.biotek.com/products/product_detail.php?pid=117 The FLx800™ Fluorescence Microplate Reader]
-The FLx800™ Fluorescence Microplate Reader has a computer software KCJunior that was installed in a computer adjacent to the reader for collection and processing of data from our samples.