Team:NTU-Singapore/Notebook/5 July 2008
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(New page: =Saturday 5 July= *Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony. *Lu Chao: minipreps for LacI-RBS 34. *...) |
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=Saturday 5 July= | =Saturday 5 July= | ||
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*Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony. | *Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony. | ||
- | + | ===Lu Chao=== | |
- | + | : minipreps for LacI-RBS 34. | |
+ | ===Hung, Lu Chao=== | ||
**Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results. | **Nanodrop to determine concentrations and purities of LacI-RBS 34 plasmids, which show good results. | ||
**Prepare digestion mixtures for gel confirmation. | **Prepare digestion mixtures for gel confirmation. | ||
- | + | ===Darius=== | |
+ | :Gel electrophoresis to check if there are LacI-RBS 34 plasmids.<br>Result: All have the 2000 kb band, the empty vector plasmid (expected). Besides that band: | ||
**There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!) | **There is 1 sample that only has 250 bp band, which is LacI-RBS 34. (Good!!) | ||
- | **There is 2 samples that have both 250 bp band and | + | **There is 2 samples that have both 250 bp band and less than 50 bp band, which can be interpreted as RBS (due to self-ligation of RBS). |
+ | **The rest only have the RBS band. | ||
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Latest revision as of 02:24, 28 October 2008
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Contents |
Saturday 5 July
Lu Chao
Hung, Lu Chao
Darius
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