Team:University of Ottawa/7 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: __TOC__ ==Today in the lab== '''Dan and Chris''' :'''Miniprep''' ::<li>Performed a miniprep on all the innocluated strains :'''Digestion''' ::<li> Digested miniprep constructs with HindIII...)
 
(One intermediate revision not shown)
Line 1: Line 1:
 +
{|######Navigational Bar#######
 +
|}
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<script src="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBar.js" type="text/javascript"></script>
 +
<link href="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarHorizontal.css" rel="stylesheet" type="text/css" />
 +
</head>
 +
 +
<body>
 +
 +
<ul id="MenuBar1" class="MenuBarHorizontal">
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa">Home</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
 +
        </ul>
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Who_we_are">Who We Are</a>
 +
            <ul>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Advisors">Advisors</a></li>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Undergrads">Undergrads</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#What_we_did">What We've Done</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Where_we.27re_from">Where We're From</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Contact_Us">Contact Us</a></li>
 +
       
 +
      </ul>
 +
  </li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
 +
      <ul>
 +
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Template">Template</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#The_Design">Design</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#References">References</a></li>
 +
      </ul>
 +
  </li>
 +
  <li><a href="http://partsregistry.org/Part:BBa_K149001:Design">BioBricks</a></li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
 +
    <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li>
 +
    </ul>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li>   
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li>       
 +
      </ul>
 +
  </li>
 +
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
<script type="text/javascript">
 +
<!--
 +
var MenuBar1 = new Spry.Widget.MenuBar("MenuBar1", {imgDown:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarDownHover.gif", imgRight:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarRightHover.gif"});
 +
//-->
 +
</script>
 +
</body>
 +
</html>
 +
__TOC__
__TOC__
==Today in the lab==
==Today in the lab==
Line 8: Line 76:
:'''Gel'''
:'''Gel'''
::<li>
::<li>
 +
'''Matt'''
 +
:'''Gel'''
 +
::<li> A gel was run for the digestion on Friday but did not turn out correctly - the correct band sizes did not appear.
 +
::<li> The same digestion was performed again, this time using a little more PstI enzyme. Gel results still gave incrrect band sizes.
 +
::<li> Performing an inoculation again with new colonies chosen from plates.

Latest revision as of 23:44, 28 October 2008

Untitled Document

 

 

Contents

Today in the lab

Dan and Chris

Miniprep
  • Performed a miniprep on all the innocluated strains
    • Digestion
  • Digested miniprep constructs with HindIII and Atcre with EcoRI
    • Gel

    • Matt

    Gel
  • A gel was run for the digestion on Friday but did not turn out correctly - the correct band sizes did not appear.
  • The same digestion was performed again, this time using a little more PstI enzyme. Gel results still gave incrrect band sizes.
  • Performing an inoculation again with new colonies chosen from plates.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/7_July_2008"