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User:University of Washington/9 July 2008
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| + | == RP4 Conjugation == | ||
| + | ·Success!!! Bacteria-Bacteria conjugation works<br\> | ||
| + | ·Received lambda red primers for RP4 manipulation | ||
| + | == LuxR from AraC and TetR == | ||
| + | |||
| + | - Took AraC plasmid after thermo cycling out from the machine, stored in the fridge. | ||
| + | |||
| + | - Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp. | ||
| + | |||
| + | == BioBrick Promoter Measurements == | ||
| + | |||
| + | - Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours. | ||
| + | |||
| + | - Using a 96-well plate reader, fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan. | ||
| + | |||
| + | ==LuxR from pLac== | ||
| + | |||
| + | -Part I736004 sequence was confirmed. | ||
| + | |||
| + | -Overnight culture of I736004 transformed cells were started in M9 media. | ||
| + | |||
| + | |||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 23:40, 24 July 2008
Contents |
RP4 Conjugation
·Success!!! Bacteria-Bacteria conjugation works
·Received lambda red primers for RP4 manipulation
LuxR from AraC and TetR
- Took AraC plasmid after thermo cycling out from the machine, stored in the fridge.
- Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp.
BioBrick Promoter Measurements
- Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours.
- Using a 96-well plate reader, fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan.
LuxR from pLac
-Part I736004 sequence was confirmed.
-Overnight culture of I736004 transformed cells were started in M9 media.
Back to Team:University_of_Washington/Notebook#Notebook
