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Team:Mississippi State/Papers
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| - | [[Team:Mississippi State| | + | [[Team:Mississippi State|MSUSB iGEM 08]] |
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| + | ===[[Team:Mississippi State/Notebook|Notebook]]=== | ||
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| + | !align="center"|{{ #calendar: title=Team:Mississippi_State |year=2008 | month=06 }} | ||
| + | !align="center"|{{ #calendar: title=Team:Mississippi_State |year=2008 | month=07 }} | ||
| + | !align="center"|{{ #calendar: title=Team:Mississippi_State |year=2008 | month=08 }} | ||
| + | !align="center"|{{ #calendar: title=Team:Mississippi_State |year=2008 | month=09 }} | ||
| + | !align="center"|{{ #calendar: title=Team:Mississippi_State |year=2008 | month=10 }} | ||
| + | !align="left"|Pichia Protocols | ||
| + | #[[Team:Mississippi State/Recipes|Recipes]] | ||
| + | #[[Team:Mississippi State/Maintenance of Plasmids|Maintenance of Plasmids]] | ||
| + | #[[Team:Mississippi State/Making Competent Cells|Making Competent Cells]] | ||
| + | #[[Team:Mississippi State/Making LB Media for Plates|Making LB Media for Plates]] | ||
| + | #[[Team:Mississippi State/Making Blasticidin Stock Solution|Making Blasticidin Stock Solution]] | ||
| + | #[[Team:Mississippi State/Transformation of pPIC6alpha into E.Coli|Transformation of pPIC6alpha into E.Coli]] | ||
| + | #[[Team:Mississippi State/Running a Gel|Running a Gel]] | ||
| + | |} | ||
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| + | </html></div> | ||
| - | + | ===Papers=== | |
| - | + | * [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=333654&blobtype=pdf Genomic Organization of Lignin Peroxidase Genes of Phanerochaete Chrysosporium] | |
| - | + | * [http://www3.interscience.wiley.com/cgi-bin/fulltext/2575/PDFSTART Breakdown of Plant Polymers by Fungi and their Potential for use in Bioremediation] | |
| - | + | * [http://old.iupac.org/publications/pac/1996/pdf/6811x2089.pdf On the Interaction of Lignin Peroxidase with Lignin] | |
| - | + | * [http://jb.asm.org/cgi/content/full/181/11/3427 Organization and Differential Regulation of a Cluster of Lignin Peroxidase Genes of Phanerochaete chrysosporium] | |
| - | + | "Why P. chrysosporium maintains multiple isozymes to catalyze presumably similar reactions remains unclear. Some substrate and kinetic differences between LiP isozymes have been observed previously (14, 20) and may indicate specific roles for individual LiPs during lignin depolymerization. Alternatively, it is possible that the majority of LiPs are redundant, having arisen through various chromosomal rearrangements such as duplications, translocations, or unequal crossover events during meiosis. The report of an insertion element that transcriptionally inactivates lipI2 indicates that not all alleles are necessary for efficient lignin depolymerization (17). In addition, there is growing evidence that redundant genes are maintained if they are not deleterious to the organism (18, 39)." | |
| - | + | * [http://www.pnas.org/cgi/reprint/89/12/5586?ijkey=752aa6d2da3eabae0fd24cd8a31ee4c30b6444db cAMP-Mediated Differential Regulation of Lignin Peroxidase and Manganese-Dependent Peroxidase Production in the White-Rot Basidiomycete Phanerochaete chrysosporium] | |
| - | + | "LIP and MNP gene expression appears to be differentially regulated depending on the intracellular concentration of cAMP. These results show that cAMP plays a key role in the regulation of production of LIPs and MNPs in P. chrysosporium." | |
| - | + | * [http://www.jbc.org/cgi/reprint/268/17/12274 Ligninolysis by a Purified Lignin Peroxidase] | |
| - | + | LiP definitely plays a major role in the initiation of Ligninolysis. The enzyme alone is capable of breaking the lignin structure, but repolymerization occurs when the proper mechanisms are not present to uptake the polymer units. Veratryl alcohol was also noted to be of high significance to LiP activity. | |
| - | + | * [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201370&pageindex=1 Biosynthetic Pathway for Veratryl Alcohol in the Ligninolytic Fungus Phanerochaete chrysosporium] | |
| + | This one shows the pathway for Valc synthesis in white rot fungi. | ||
[[Image:Beaver.JPG|300 px|center]] | [[Image:Beaver.JPG|300 px|center]] | ||
Latest revision as of 19:05, 28 October 2008
| Team | Project | Papers and Notebook |
Notebook
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Papers
- [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=333654&blobtype=pdf Genomic Organization of Lignin Peroxidase Genes of Phanerochaete Chrysosporium]
- [http://www3.interscience.wiley.com/cgi-bin/fulltext/2575/PDFSTART Breakdown of Plant Polymers by Fungi and their Potential for use in Bioremediation]
- [http://old.iupac.org/publications/pac/1996/pdf/6811x2089.pdf On the Interaction of Lignin Peroxidase with Lignin]
- [http://jb.asm.org/cgi/content/full/181/11/3427 Organization and Differential Regulation of a Cluster of Lignin Peroxidase Genes of Phanerochaete chrysosporium]
"Why P. chrysosporium maintains multiple isozymes to catalyze presumably similar reactions remains unclear. Some substrate and kinetic differences between LiP isozymes have been observed previously (14, 20) and may indicate specific roles for individual LiPs during lignin depolymerization. Alternatively, it is possible that the majority of LiPs are redundant, having arisen through various chromosomal rearrangements such as duplications, translocations, or unequal crossover events during meiosis. The report of an insertion element that transcriptionally inactivates lipI2 indicates that not all alleles are necessary for efficient lignin depolymerization (17). In addition, there is growing evidence that redundant genes are maintained if they are not deleterious to the organism (18, 39)."
- [http://www.pnas.org/cgi/reprint/89/12/5586?ijkey=752aa6d2da3eabae0fd24cd8a31ee4c30b6444db cAMP-Mediated Differential Regulation of Lignin Peroxidase and Manganese-Dependent Peroxidase Production in the White-Rot Basidiomycete Phanerochaete chrysosporium]
"LIP and MNP gene expression appears to be differentially regulated depending on the intracellular concentration of cAMP. These results show that cAMP plays a key role in the regulation of production of LIPs and MNPs in P. chrysosporium."
- [http://www.jbc.org/cgi/reprint/268/17/12274 Ligninolysis by a Purified Lignin Peroxidase]
LiP definitely plays a major role in the initiation of Ligninolysis. The enzyme alone is capable of breaking the lignin structure, but repolymerization occurs when the proper mechanisms are not present to uptake the polymer units. Veratryl alcohol was also noted to be of high significance to LiP activity.
- [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201370&pageindex=1 Biosynthetic Pathway for Veratryl Alcohol in the Ligninolytic Fungus Phanerochaete chrysosporium]
This one shows the pathway for Valc synthesis in white rot fungi.
