Latest revision as of 03:36, 12 July 2008

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Things we did today

Grace

The two expected bands corresponding to the vector and the BioBrick insert are clearly visible. First "good" plasmid prep!

Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen.
Redid ligation and RE digest. Loaded 20 μl in 4% gel.
RE digested C0012.
Gel extraction of C0012 vector and ligated products.
Ligated C0012 vector with pnir, slr2016, and pilA.

Krystle

Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells.

Margaret

* Ran a gel of the rep and mob region.

!

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Completed oligonucleotide experiment===
 :<strong> Grace</strong>
+[[Image:C0012_plasmid_prep.JPG|thumb|right|The two expected bands corresponding to the vector and the BioBrick insert are clearly visible. First "good" plasmid prep!]]
 :* Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen.
 :* Redid ligation and RE digest. Loaded 20 &mu;l in 4% gel.
@@ Line 16: / Line 16: @@
 :* Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5&alpha; super competent cells.
+===PCR of pRL1383a parts===
+:<strong> Margaret </strong>
+        * Ran a gel of the rep and mob region. [[Image:mob_rep_gel.JPG|thumb|right|!]]
-[[Image:C0012_plasmid_prep.JPG|thumb|right|The two expected bands corresponding to the vector and the BioBrick insert are clearly visible.]]
-===Restriction Digest of C0012 plasmid prep===
-:<strong> Grace</strong>
 = Discussion =