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Team:The University of Alberta/11 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: Chris is continuing Jason's BO/J6 stuff, refer to flow chart on the board... Yesterday's transformation is weird, the "digest" plate has lots of growth...huh, maybe our enzyme is not worki...) |
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| - | Chris | + | '''Chris''' <br> |
| - | Yesterday's transformation is weird, the "digest" plate has lots of growth...huh, maybe our enzyme is not working good enough | + | *Sequenced inserts of Blue Ox in J61003 Transf.2 and Transf.4. Will run them up to MBSU for sequencing on Monday. |
| - | Winnie | + | *Set up O/N cultures of Tansf.2 and Transf.4 for minipreps; volunteers will miniprep tomorrow. |
| - | did blast on sequencing J61003 with 23, 25, 35 and 99 | + | |
| - | except 99 everything matched, | + | '''Tom'''<br> |
| - | sequenced Tom's I0500 +35 and 99 using reverse primer | + | *Yesterday's transformation is weird, the "digest" plate has lots of growth...huh, maybe our enzyme is not working good enough |
| - | made LB+kan plates. Volunteers on saturday, please put them in the -4c | + | |
| - | took pictures of gel for Tom and Chris, Tom's gel is in the firdge. | + | '''Winnie'''<br> |
| - | Chris's gel is not as expected, so left in the fridge till Monday to ask Chris. | + | *did blast on sequencing J61003 with 23, 25, 35 and 99 |
| + | except 99 everything matched,yes! | ||
| + | *sequenced Tom's I0500 +35 and 99 using reverse primer | ||
| + | *made LB+kan plates. Volunteers on saturday, please put them in the -4c | ||
| + | *took pictures of gel for Tom and Chris, Tom's gel is in the firdge. | ||
| + | **Chris's gel is not as expected, so left in the fridge till Monday to ask Chris. '''EDIT''': Gel was fine! | ||
Latest revision as of 18:14, 15 July 2008
Chris
- Sequenced inserts of Blue Ox in J61003 Transf.2 and Transf.4. Will run them up to MBSU for sequencing on Monday.
- Set up O/N cultures of Tansf.2 and Transf.4 for minipreps; volunteers will miniprep tomorrow.
Tom
- Yesterday's transformation is weird, the "digest" plate has lots of growth...huh, maybe our enzyme is not working good enough
Winnie
- did blast on sequencing J61003 with 23, 25, 35 and 99
except 99 everything matched,yes!
- sequenced Tom's I0500 +35 and 99 using reverse primer
- made LB+kan plates. Volunteers on saturday, please put them in the -4c
- took pictures of gel for Tom and Chris, Tom's gel is in the firdge.
- Chris's gel is not as expected, so left in the fridge till Monday to ask Chris. EDIT: Gel was fine!
