Team:NTU-Singapore/Notebook/12 July 2008
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(New page: =Saturday 12 July= ==Lab details== *'''Choon Kit''' **'''Inoculation of colonies that were cloned on [https://2008.igem.org/Team:NTU-Singapore/Notebook/11_July_2008?title= 11 July 2008]'''...) |
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=Saturday 12 July= | =Saturday 12 July= | ||
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==Lab details== | ==Lab details== | ||
*'''Choon Kit''' | *'''Choon Kit''' | ||
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***A transformation and cloning into home-made Top10 competent cells were carried out. Instead of the usual practice to plate 250ul of LB/bacteria culture into one single LBA plate, it was divided into 2 plates, each containing approximately 125ul of bacteria culture. This modification was to encourage larger colony growth and enable selection at the follow up step easier. Cells that were given larger space to grow will develop into larger colonies. | ***A transformation and cloning into home-made Top10 competent cells were carried out. Instead of the usual practice to plate 250ul of LB/bacteria culture into one single LBA plate, it was divided into 2 plates, each containing approximately 125ul of bacteria culture. This modification was to encourage larger colony growth and enable selection at the follow up step easier. Cells that were given larger space to grow will develop into larger colonies. | ||
***Action to be followed up: Inoculation of E7-AmpR vector and T7ptag-AmpR vector. | ***Action to be followed up: Inoculation of E7-AmpR vector and T7ptag-AmpR vector. | ||
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Latest revision as of 02:27, 28 October 2008
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Saturday 12 July
Lab details
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