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Team:ESBS-Strasbourg/14 July 2008
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(New page: < back == DryLab == === Modeling === == WetLab == results from the transformation * 99 colonies of the control plasmid in TOP10(invitrogen), 5*10^6 cf...) |
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[[Team:ESBS-Strasbourg/Notebook | < back]] | [[Team:ESBS-Strasbourg/Notebook | < back]] | ||
== DryLab == | == DryLab == | ||
| + | |||
| + | === Wiki === | ||
| + | *Navigation and Header (MM) | ||
| + | *Getting stucked in IRESes: the ccdIRES works only in humans... | ||
=== Modeling === | === Modeling === | ||
| Line 8: | Line 12: | ||
* 99 colonies of the control plasmid in TOP10(invitrogen), 5*10^6 cfu/ug (still not enough) | * 99 colonies of the control plasmid in TOP10(invitrogen), 5*10^6 cfu/ug (still not enough) | ||
* 1 colonie of the BBa_J63002(Adh1_ter) in TOP10(invitrogen), PCR with primers <<verification pSB1A3 left/right>> should deliver a 461 bp fragment | * 1 colonie of the BBa_J63002(Adh1_ter) in TOP10(invitrogen), PCR with primers <<verification pSB1A3 left/right>> should deliver a 461 bp fragment | ||
| + | <br> | ||
| + | *Media | ||
| + | ** bacteria: LB-medium (500 ml), LB-medium with Ampicillin (250 ml), LB-Agar-plates without antibiotics (7) | ||
| + | ** yeast: YPD-medium (500 ml) | ||
| + | <br> | ||
| + | *Overnight cultures | ||
| + | ** bacteria: | ||
| + | ** yeast: strain BYA4742 (28°C) | ||
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 16:52, 16 July 2008
Contents |
DryLab
Wiki
- Navigation and Header (MM)
- Getting stucked in IRESes: the ccdIRES works only in humans...
Modeling
WetLab
results from the transformation
- 99 colonies of the control plasmid in TOP10(invitrogen), 5*10^6 cfu/ug (still not enough)
- 1 colonie of the BBa_J63002(Adh1_ter) in TOP10(invitrogen), PCR with primers <<verification pSB1A3 left/right>> should deliver a 461 bp fragment
- Media
- bacteria: LB-medium (500 ml), LB-medium with Ampicillin (250 ml), LB-Agar-plates without antibiotics (7)
- yeast: YPD-medium (500 ml)
- Overnight cultures
- bacteria:
- yeast: strain BYA4742 (28°C)
