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Team:ESBS-Strasbourg/Dephosphorylation
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | a) Remarks <br> | |
Do the reaction directly in the RE mix <br> | Do the reaction directly in the RE mix <br> | ||
| Line 5: | Line 5: | ||
| - | + | b) Protocol <br> | |
-Add 2 unit of CIP for 2µg of vector <br> | -Add 2 unit of CIP for 2µg of vector <br> | ||
| Line 12: | Line 12: | ||
| - | [[Team:ESBS-Strasbourg/Protocols|Protocols]] | + | [[Team:ESBS-Strasbourg/Protocols|''Protocols'']] |
Latest revision as of 14:30, 14 July 2008
a) Remarks
Do the reaction directly in the RE mix
b) Protocol
-Add 2 unit of CIP for 2µg of vector
-Digestion 1h max. at 37°C
