Wisconsin: Lignin Project/19 June 2008

From 2008.igem.org

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(New page: '''Team Fungus:''' <br> ---- Took spec reading of Carbon and Nitrogen-limited cDNA<br> Worked on Wiki site<br> Worked on sponsorship<br> Made overnight cultures of: <br> BL21 (x2)<br> pB...)
 
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'''Team Fungus:''' <br>
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<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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|'''Team Sorbitol:'''
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Purified the PCR product from yesterday - not enough DNA for a digestion<br>
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Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands)<br>
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In the meantime designed a digestion reaction to use once we get enough product.<br>
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Ran model simulations of knockouts in program. Interesting knockout:
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TpiA~Triosephosphate isomerase 
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E.coli K12 MG1655, locus tag b3919, GeneID 948409, EC 5.3.1.1
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Obtained TpiA knockout from Keio Collection plated on LB+Kan
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Determined best media formulation C Media supplemented with glycerol<br>
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'''Team Fungus:''' <br>
Took spec reading of Carbon and Nitrogen-limited cDNA<br>
Took spec reading of Carbon and Nitrogen-limited cDNA<br>
Worked on Wiki site<br>
Worked on Wiki site<br>
Worked on sponsorship<br>
Worked on sponsorship<br>
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Made overnight cultures of: <br>
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Made overnight cultures of: BL21 (x2), pBad33, pMcSG7, pet28a, pet28b, DH1 (x2), pBad21<br>
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BL21 (x2)<br>
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Made and autoclaved more LB solution for future use <br>
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pBad33<br>
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Designed new and longer primers <br>
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pMcSG7<br>
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|}
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pet28a<br>
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pet28b<br>
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DH1 (x2)<br>
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pBad21<br>
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Latest revision as of 17:20, 18 October 2008

Igemwibanner.gif
Team Sorbitol:

Purified the PCR product from yesterday - not enough DNA for a digestion
Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands)
In the meantime designed a digestion reaction to use once we get enough product.
Ran model simulations of knockouts in program. Interesting knockout: TpiA~Triosephosphate isomerase E.coli K12 MG1655, locus tag b3919, GeneID 948409, EC 5.3.1.1 Obtained TpiA knockout from Keio Collection plated on LB+Kan Determined best media formulation C Media supplemented with glycerol
Team Fungus:
Took spec reading of Carbon and Nitrogen-limited cDNA
Worked on Wiki site
Worked on sponsorship
Made overnight cultures of: BL21 (x2), pBad33, pMcSG7, pet28a, pet28b, DH1 (x2), pBad21
Made and autoclaved more LB solution for future use
Designed new and longer primers

Retrieved from "http://2008.igem.org/Wisconsin:_Lignin_Project/19_June_2008"