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Wisconsin: Lignin Project/25 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:'''<br> | ||
| + | Attended the Wisconsin iGEM team meeting<br> | ||
| + | Re-evaluated our PCR reactions and primers<br> | ||
| + | Decided to alter the temperatures and times of several steps.<br> | ||
| + | Also we redesigned the primers used to clone out the ''srl'' operon.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
| - | + | Digested PCR product and pET28a vector<br> | |
| - | Ran gel of digestion and performed gel purification<br> | + | Ran 1% agrose gel of digestion and performed gel purification<br> |
| - | - | + | -digested and undigested PCR product as well as digested vector showed correctly sized bands <br> |
| - | + | -undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder <br> | |
| + | Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit | ||
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Latest revision as of 15:25, 8 August 2008
| Team Sorbitol: Attended the Wisconsin iGEM team meeting Team Fungus: |
