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Wisconsin: Lignin Project/27 June 2008
From 2008.igem.org
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(New page: '''Team Fungus:''' <br> Plate with 1:3 insert to vector ratio had 2 colonies<br> Plate with 0:1 vector had 1 colony<br> Rest had zero colonies<br> We streaked and plated the two colonies f...) |
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| + | |'''Team Sorbitol:''' | ||
| + | Checked the PCR product from last night on a gel and found several bands to work again (700bp).<br> | ||
| + | The product and pBAD30 were digested with Xba1 and HindIII restriction enzymes.<br> | ||
| + | They were verified to work on a gel and extracted using a Qiagen Gel Extraction Kit.<br> | ||
| + | Unfortunately not enough DNA was obtained to perform a ligation.<br> | ||
| + | Overnight culture started using revised C-Media recipe: growth after overnight incubation at 30C was observed<br> | ||
| + | Single colony overnight culture started for growth curve following morning.<br> | ||
'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Plate with 1:3 insert to vector ratio had 2 colonies<br> | Plate with 1:3 insert to vector ratio had 2 colonies<br> | ||
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We streaked and plated the two colonies from the 1:3 onto two new LB + Kan plates<br> | We streaked and plated the two colonies from the 1:3 onto two new LB + Kan plates<br> | ||
Made more LB + Kan plates<br> | Made more LB + Kan plates<br> | ||
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Latest revision as of 19:49, 18 October 2008
| Team Sorbitol:
Checked the PCR product from last night on a gel and found several bands to work again (700bp). |
