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Wisconsin: Lignin Project/16 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:''' | ||
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| + | Harvested the pBAD30 and pBAD18 from the cells using a Qiagen Miniprep Kit. | ||
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| + | Stored this DNA in a -20 Freezer | ||
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| + | Began cultures of MG1655 to use for cloning. | ||
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| + | Experimented with different media recipes for optimal growth. | ||
| + | M9 minimal media with: 1.) 20%glucose +++, 2.) 20% glycerol++, 3.) 20%acetate+ | ||
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| + | Met with Jennie Reed, modeling work adding sorbitol to basic cell model | ||
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'''Team Fungus:'''<br> | '''Team Fungus:'''<br> | ||
Made LB + Kanamycin plates <br> | Made LB + Kanamycin plates <br> | ||
Streaked out vector (pet28a) on LB + Kanamycin plates <br> | Streaked out vector (pet28a) on LB + Kanamycin plates <br> | ||
Made dilute Kanamycin freezer aliquots <br> | Made dilute Kanamycin freezer aliquots <br> | ||
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Latest revision as of 16:35, 18 October 2008
| Team Sorbitol:
Harvested the pBAD30 and pBAD18 from the cells using a Qiagen Miniprep Kit. Stored this DNA in a -20 Freezer Began cultures of MG1655 to use for cloning. Experimented with different media recipes for optimal growth. M9 minimal media with: 1.) 20%glucose +++, 2.) 20% glycerol++, 3.) 20%acetate+ Met with Jennie Reed, modeling work adding sorbitol to basic cell model Team Fungus: |
