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Wisconsin: Lignin Project/23 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:''' | ||
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| + | Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity)<br> | ||
| + | Ran a new PCR reaction using the specific volumes designated by the Herculase designers.<br> | ||
| + | No Bands formed.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
| - | Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA<br> | + | Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase<br> |
| - | Ran PCR products through 0.9% agrose gel at 100V | + | Ran PCR products through 0.9% agrose gel at 100V: bands present for both at about 1.1kb <br> |
| - | + | Grew overnight culture of pET28a <br> | |
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Latest revision as of 15:21, 8 August 2008
| Team Sorbitol:
Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity) Team Fungus: |
