Team:NTU-Singapore/Notebook/15 July 2008
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(New page: *All 4 plates of Fe-GFP from Monday had colonies. We did the inoculation in the afternoon. *Ligation of T7ptag, E7 with empty vector from GFP: **We already got E7 (cut with E/P), E7 (cut w...) |
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+ | =Tuesday 15 July= | ||
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*All 4 plates of Fe-GFP from Monday had colonies. We did the inoculation in the afternoon. | *All 4 plates of Fe-GFP from Monday had colonies. We did the inoculation in the afternoon. | ||
*Ligation of T7ptag, E7 with empty vector from GFP: | *Ligation of T7ptag, E7 with empty vector from GFP: | ||
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*1725-1930: transform of the succesfully ligated LacI-RBS34 and LacI-GFP plasmids into top10 cells. | *1725-1930: transform of the succesfully ligated LacI-RBS34 and LacI-GFP plasmids into top10 cells. | ||
*Inoculation of Fe-GFP (3 colonies each plate x 4 plates); GFP (6 colonies). | *Inoculation of Fe-GFP (3 colonies each plate x 4 plates); GFP (6 colonies). | ||
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Latest revision as of 02:30, 28 October 2008
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Tuesday 15 July
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