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Team:The University of Alberta/15 July 2008
From 2008.igem.org
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(New page: ===Today=== *Got sequencing back from Blue Ox in J61003 Transf.2 and Transf. 4. Both of them look good; Transf.2 looks "better" though because it has fewer mismatches. *Did PCR on Tryp aga...) |
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*Got sequencing back from Blue Ox in J61003 Transf.2 and Transf. 4. Both of them look good; Transf.2 looks "better" though because it has fewer mismatches. | *Got sequencing back from Blue Ox in J61003 Transf.2 and Transf. 4. Both of them look good; Transf.2 looks "better" though because it has fewer mismatches. | ||
*Did PCR on Tryp again because no one can remember if it had been done successfully with the newest primers. | *Did PCR on Tryp again because no one can remember if it had been done successfully with the newest primers. | ||
| + | **Ran portion of PCR out on 1% gel to confirm product. PCR purified the rest. | ||
| + | *Transformed the I0500+new vector ligation | ||
| + | *The BioBricks we had ordered for synthesis have arrived (minus one of them)! Siama has transformed them following the protocol supplied by the company. | ||
Latest revision as of 21:26, 16 July 2008
Today
- Got sequencing back from Blue Ox in J61003 Transf.2 and Transf. 4. Both of them look good; Transf.2 looks "better" though because it has fewer mismatches.
- Did PCR on Tryp again because no one can remember if it had been done successfully with the newest primers.
- Ran portion of PCR out on 1% gel to confirm product. PCR purified the rest.
- Transformed the I0500+new vector ligation
- The BioBricks we had ordered for synthesis have arrived (minus one of them)! Siama has transformed them following the protocol supplied by the company.
