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User:University of Washington/15 July 2008
From 2008.igem.org
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·pAC88 | ·pAC88 | ||
| - | Bac-Bac conjugation [[Protocols| protocol #1]]<br> | + | Bac-Bac conjugation [[Team:University_of_Washington/Protocols| protocol #1]]<br> |
·DH5alpha + RP4<br> | ·DH5alpha + RP4<br> | ||
·DH5alpha + pCS26-pac | ·DH5alpha + pCS26-pac | ||
| + | |||
| + | == LuxR from AraC and TetR == | ||
| + | |||
| + | - Miniprepped 12 overnight culture of mutated AraC into 30 ul EB | ||
| + | |||
| + | - Ordered sequences of all 12: 8 ul (1 umol/mL) forward primer + 4 ul DNA | ||
| + | |||
| + | - Grew 4 overnight culture of D29 from Elowitz's lab | ||
| + | |||
| + | ==LuxR from pLac== | ||
| + | |||
| + | - Parts R0010, E0420, and I0462 were digested, ran on gel, then extracted for assembly of two new parts: R0010 in front of E0420, and R0010 in front of I0462. Biocircuits lab protocol used. | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 21:52, 25 July 2008
RP4 Conjugation
Mini prep
·pAC88
Bac-Bac conjugation protocol #1
·DH5alpha + RP4
·DH5alpha + pCS26-pac
LuxR from AraC and TetR
- Miniprepped 12 overnight culture of mutated AraC into 30 ul EB
- Ordered sequences of all 12: 8 ul (1 umol/mL) forward primer + 4 ul DNA
- Grew 4 overnight culture of D29 from Elowitz's lab
LuxR from pLac
- Parts R0010, E0420, and I0462 were digested, ran on gel, then extracted for assembly of two new parts: R0010 in front of E0420, and R0010 in front of I0462. Biocircuits lab protocol used.
Back to Team:University_of_Washington/Notebook#Notebook
