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Team:ESBS-Strasbourg/16 July 2008
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[[Team:ESBS-Strasbourg/Notebook | < back]] | [[Team:ESBS-Strasbourg/Notebook | < back]] | ||
== DryLab == | == DryLab == | ||
| + | |||
| + | michi: | ||
| + | * IRESes s*** let's mutate our promoter | ||
| + | * last chance for IRES: wrote email to Pospisek | ||
| + | * Calculated size of our constructs: 6 kb (too big for vector?) | ||
| + | |||
| + | === Wiki === | ||
| + | * Further content in all categories (MM) | ||
=== Modeling === | === Modeling === | ||
| Line 7: | Line 15: | ||
Sandra: | Sandra: | ||
* Gel of the PCR from yesterday | * Gel of the PCR from yesterday | ||
| + | * Transformations | ||
| + | **'''cells | part/vector | protocol''' | ||
| + | <pre> | ||
| + | dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria) | ||
| + | = | = | KCM (Barbara) | ||
| + | = | LexA-BD (1 µl) | = | ||
| + | = | BB_pSB (2 µl) | = | ||
| + | = | BB_1763004 (2 µl)| = | ||
| + | dh5a (ours) | pxj (10 ng) | HS | ||
| + | Top10 | = | = | ||
| + | db3.1 | = | = | ||
| + | = | BB_p1010 (2 µl) | = | ||
| + | </pre> | ||
<br> | <br> | ||
Mariel repeats the PCR from Paul from yesterday with 10ng plasmids <br> | Mariel repeats the PCR from Paul from yesterday with 10ng plasmids <br> | ||
=>Amplification of Gal4 succeed | =>Amplification of Gal4 succeed | ||
| + | |||
| + | Paul: | ||
| + | * Purification of Gal4 PCR product, stored at -20°C | ||
| + | <br> | ||
| + | Katja: | ||
| + | *Preparation of TENS buffer (not steril filtered) | ||
| + | *Preparation of 3M NaAcO pH5 | ||
| + | *Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR failed | ||
== General == | == General == | ||
| + | Our account is open again.(KK) | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 10:11, 17 July 2008
Contents |
DryLab
michi:
- IRESes s*** let's mutate our promoter
- last chance for IRES: wrote email to Pospisek
- Calculated size of our constructs: 6 kb (too big for vector?)
Wiki
- Further content in all categories (MM)
Modeling
WetLab
Sandra:
- Gel of the PCR from yesterday
- Transformations
- cells | part/vector | protocol
dh5a (Mariel) | pxj (10 ng) | HeatShock (Maria)
= | = | KCM (Barbara)
= | LexA-BD (1 µl) | =
= | BB_pSB (2 µl) | =
= | BB_1763004 (2 µl)| =
dh5a (ours) | pxj (10 ng) | HS
Top10 | = | =
db3.1 | = | =
= | BB_p1010 (2 µl) | =
Mariel repeats the PCR from Paul from yesterday with 10ng plasmids
=>Amplification of Gal4 succeed
Paul:
- Purification of Gal4 PCR product, stored at -20°C
Katja:
- Preparation of TENS buffer (not steril filtered)
- Preparation of 3M NaAcO pH5
- Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR failed
General
Our account is open again.(KK)
