21 May 2008

From 2008.igem.org

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==Volunteers Scheduled for Today==
 
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5-8 AS<br>
 
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6-8 DL<br>
 
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Things to do:<br>
 
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Mini-preps of O/N's<br>
 
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Sequence gel purified fragments from yesterday<br>
 
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Transform I0500<br>
 
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Digest J61003 with Xba/Pst to insert ORF's<br>
 
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Digest minipreps with Xba/Pst to insert into J61003<br>
 
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Run gel of digests and excise correct fragments<br>
 
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Ligate vector/inserts<br>
 
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Transform ligation reactions<br>
 
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Lab clean up<br>
 

Latest revision as of 19:20, 22 May 2008