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Team:MIT/Notebook
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!align="center"|[[Team:MIT/Team|The Team]] | !align="center"|[[Team:MIT/Team|The Team]] | ||
!align="center"|[[Team:MIT/Project|The Project]] | !align="center"|[[Team:MIT/Project|The Project]] | ||
| + | !align="center"|[[Team:MIT/Experiments|Experiments]] | ||
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]] | !align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]] | ||
| - | !align="center"|[[Team:MIT/Modeling| | + | !align="center"|[[Team:MIT/Modeling|Results]] |
!align="center"|[[Team:MIT/Notebook|Notebook]] | !align="center"|[[Team:MIT/Notebook|Notebook]] | ||
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*[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks Preparing Antibiotic Stocks] | *[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks Preparing Antibiotic Stocks] | ||
*[[Team:MIT/Protein_Purification|Protein Purification]] | *[[Team:MIT/Protein_Purification|Protein Purification]] | ||
| - | * [[Team:MIT/Tooth_binding_assay_protocol|Tooth | + | * [[Team:MIT/Tooth_binding_assay_protocol|Novel Tooth Binding Assay]] |
| + | * [[Team:MIT/Transforming_Lactococcus lactis|Lactococcus lactis Transformation Protocol]] | ||
| + | *[[Team:MIT/Colony_PCR|Colony PCR]] | ||
== Data == | == Data == | ||
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*PMID 12788739<br style="clear:both;"/> Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).<br style="clear:both;"/> | *PMID 12788739<br style="clear:both;"/> Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).<br style="clear:both;"/> | ||
*PMID 11772607<br style="clear:both;"/> Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. | *PMID 11772607<br style="clear:both;"/> Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. | ||
| + | *High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol [http://www.biomedcentral.com/1472-6750/7/15] | ||
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!align="center"|[[Team:MIT/Team|The Team]] | !align="center"|[[Team:MIT/Team|The Team]] | ||
!align="center"|[[Team:MIT/Project|The Project]] | !align="center"|[[Team:MIT/Project|The Project]] | ||
| + | !align="center"|[[Team:MIT/Experiments|Experiments]] | ||
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]] | !align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]] | ||
!align="center"|[[Team:MIT/Modeling|Modeling]] | !align="center"|[[Team:MIT/Modeling|Modeling]] | ||
Latest revision as of 14:25, 29 October 2008
| Home | The Team | The Project | Experiments | Parts Submitted to the Registry | Results | Notebook |
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Procedural Daily Notebook
- Click on any day below to see what wet-lab procedures were conducted.
Organizational Daily Notebook
- Please see our daily notebook for a detailed daily summary of our daily meetings, brainstorming, discussions, and progress.
Information
- Primers/Plasmids/PCR sequences
- Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols
- Vocabulary List
Protocols
- [http://openwetware.org/wiki/MIT_iGEM_T4_and_Quick_Ligation DNA Ligations]
- [http://openwetware.org/wiki/MIT_iGEM_Top10_ChemComp_Ecoli_transformation_protocol#Regular_Transformation_protocol_for_Top10_cells Top10 chem competent cell transformation]
- [http://openwetware.org/wiki/Knight:Electroporation BLR electrocompetent cell transformation]
- [http://web.mit.edu/biopolymers/www/DNA.html DNA sequencing]
- [http://openwetware.org/wiki/Endy_pcr PCR]
- [http://openwetware.org/wiki/MIT_iGEM_Agarose_Gels Running DNA Gels]
- [http://openwetware.org/wiki/MIT_iGEM_Restriction_Digests Restriction Digests]
- [http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks Preparing Antibiotic Stocks]
- Protein Purification
- Novel Tooth Binding Assay
- Lactococcus lactis Transformation Protocol
- Colony PCR
Data
Useful Links
- [http://www.biology.utah.edu/jorgensen/wayned/ape/ DNA Plasmid Editor]
- [http://slam.bs.jhmi.edu/gd/ Peptide Sequence Manipulations]
- Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
- PMID 9920267
A synthetic peptide adhesion epitope as a novel antimicrobial agent.
- PMID 9062560
Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
- PMID 4055036
Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles.
- PMID 12788739
Determination of the domain of the Lactobacillus delbrueckii subsp. bulgaricus cell surface proteinase PrtB involved in attachment to the cell wall after heterologous expression of the prtB gene in Lactococcus lactis. (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
- PMID 11772607
Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. - High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol [http://www.biomedcentral.com/1472-6750/7/15]
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