Team:NTU-Singapore/Notebook/24 July 2008
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(New page: =Thursday 24 July= *RESULT from Wednesday ligation and transformation: **E7-Term got 7 colonies!! **Lysis-standard vector and LsrA-standard vector got lots of colonies!! **Lysis-Term also...) |
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=Thursday 24 July= | =Thursday 24 July= | ||
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*RESULT from Wednesday ligation and transformation: | *RESULT from Wednesday ligation and transformation: | ||
**E7-Term got 7 colonies!! | **E7-Term got 7 colonies!! | ||
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*Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.<br>'''RESULT:''' | *Digestional gel check for Lysis-Terminator (with Lysis and Term. for comparision) again (this time, we used 2% gel and 100bp ladder). We tried digesting for 1 hour, and 2 hours.<br>'''RESULT:''' | ||
**Digesting for 1 hour and 2 hour didn't make much differences. | **Digesting for 1 hour and 2 hour didn't make much differences. | ||
- | ** | + | **All 3 Lysis-Term bands were actually just Term. So all the 3 colonies we obtained just contained the uncut Terminator.<br>'''NOTE:''' |
+ | ***For 2% gel, we should run for at least 1 hour in order to see clear and distinct bands. | ||
+ | ***Terminator vector is 3kb. (as contain both AmpR and KanR), not 2kb as presumed. | ||
+ | ***Bluescript vector is 2.5kb. | ||
*Inoculation: of the colonies we obtained this morning. | *Inoculation: of the colonies we obtained this morning. | ||
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Latest revision as of 02:33, 28 October 2008
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Thursday 24 July
Hung,Darius:
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