Team:BCCS-Bristol/Calendar-Notebook/1 July 2008

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<html><link rel="stylesheet" href="http://homepage.mac.com/tgorochowski/iGEM/bccs-igem.css" type="text/css"></html>
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__NOTOC__
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<div class="bccsNavBar">
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{|  align="center"
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!align="center"|[[Team:BCCS-Bristol|Home]]
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!align="center"|[[Team:BCCS-Bristol/Team|The Team]]
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!align="center"|[[Team:BCCS-Bristol/Project|The Project]]
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!align="center"|[[Team:BCCS-Bristol/Parts|Submitted Parts]]
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!align="center"|[[Team:BCCS-Bristol/Modeling|Modelling]]
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!align="center"|[[Team:BCCS-Bristol/Notebook|Wet Lab]]
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!align="center"|[[Team:BCCS-Bristol/Calendar|Calendar]]
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!align="center"|[[Team:BCCS-Bristol/Misc|Miscellaneous]]
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|}
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<br>
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</div>
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This is a page for any solutions
This is a page for any solutions
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Media for swimming agar assay plates'''
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| align="center" style="background:#f0f0f0;"|'''0.3% 400ml'''
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| align="center" style="background:#f0f0f0;"|'''0.15% 150ml'''
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| align="center" style="background:#f0f0f0;"|'''0.5% 150ml'''
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|-
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| ||||||
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|-
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| Bactotryptone||4g||1.5g||1.5g
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|-
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| NaCl||2g||0.75g||0.75g
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|-
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| BactoAgar||1.2g||0.225g||0.75g
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|-
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|
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|}
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in addition did  a streak out for master colonies of MG1655 in LB from table below overnight at 37oC
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''10ml LB'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Yeast Extract||0.05
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|-
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| Tryptone||0.1
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|-
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| Nacl||0.1
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|-
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|
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|}
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''LB Broth 100ml'''
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| align="center" style="background:#f0f0f0;"|'''weight (g)'''
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|-
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| Yeast Extract||0.5
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|-
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| Tryptone||1
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|-
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| NaCl||1
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|-
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|
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|}
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SOC medium in preparation for Biobrick transformation this consisted of
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Trypton'''
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| align="center" style="background:#f0f0f0;"|'''2g'''
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|-
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| Yeast Extract||0.5g
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|-
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| NaCl||0.05g
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|-
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|
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|}
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in 95ml of water, then autoclaved. after this add this:
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''MgCl2'''
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| align="center" style="background:#f0f0f0;"|'''0.5ml 2M solution'''
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|-
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| Glucose||2ml 1M
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|-
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|
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|}
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then freeze at -20<sup>o</sup>C. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
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'''Swimming Medium'''
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''In 100ml of water'''
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| align="center" style="background:#f0f0f0;"|'''weight (g)'''
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|-
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| Potassium Phosphate||0.1742
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|-
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| NaCl||0.392
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|-
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| Glucose||0.18016
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|-
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| EDTA||0.003722
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|-
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| Tween -20 aka polysorbate 20||0.2 µl
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|-
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|
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|}

Latest revision as of 09:50, 14 September 2008

Home The Team The Project Submitted Parts Modelling Wet Lab Calendar Miscellaneous


This is a page for any solutions

Media for swimming agar assay plates 0.3% 400ml 0.15% 150ml 0.5% 150ml
Bactotryptone4g1.5g1.5g
NaCl2g0.75g0.75g
BactoAgar1.2g0.225g0.75g

in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC

10ml LB '
Yeast Extract0.05
Tryptone0.1
Nacl0.1
LB Broth 100ml weight (g)
Yeast Extract0.5
Tryptone1
NaCl1


SOC medium in preparation for Biobrick transformation this consisted of

Trypton 2g
Yeast Extract0.5g
NaCl0.05g

in 95ml of water, then autoclaved. after this add this:

MgCl2 0.5ml 2M solution
Glucose2ml 1M

then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.


Swimming Medium

In 100ml of water weight (g)
Potassium Phosphate0.1742
NaCl0.392
Glucose0.18016
EDTA0.003722
Tween -20 aka polysorbate 200.2 µl
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