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Team:ESBS-Strasbourg/24 July 2008
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(New page: < back == DryLab == === Modeling === == WetLab == ''Sandra'' * Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good qualitiy (ratio 260...) |
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== WetLab == | == WetLab == | ||
| + | ''Manuel'' | ||
| + | *tetR, lacI PCR on BB (Primers, VFPrimers) | ||
| + | *Pfu Pol PCR on pGEX lacI containing plasmid | ||
| + | *ON of BB: | ||
| + | **J63002 | ||
| + | **J63003 | ||
| + | **J63005 | ||
| + | **J63008 | ||
''Sandra'' | ''Sandra'' | ||
| - | * Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good | + | * Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)<br> |
=> find them in the -20°C fridge in our lab in tubes with different concentrations | => find them in the -20°C fridge in our lab in tubes with different concentrations | ||
| + | |||
| + | ''Paul'' | ||
| + | Digestion of tetR/lacI/c1 plasmids, in order to check if the biobricks are really there. Used of EcoR1 and Spe1.<br> | ||
| + | |||
| + | => no results, probably not enough DNA for the digestion. To redo next day. <br> | ||
| + | |||
== General == | == General == | ||
| + | * Animation (MM) | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 13:59, 28 July 2008
Contents |
DryLab
Modeling
WetLab
Manuel
- tetR, lacI PCR on BB (Primers, VFPrimers)
- Pfu Pol PCR on pGEX lacI containing plasmid
- ON of BB:
- J63002
- J63003
- J63005
- J63008
Sandra
- Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)
=> find them in the -20°C fridge in our lab in tubes with different concentrations
Paul
Digestion of tetR/lacI/c1 plasmids, in order to check if the biobricks are really there. Used of EcoR1 and Spe1.
=> no results, probably not enough DNA for the digestion. To redo next day.
General
- Animation (MM)
