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Alberta NINT/22 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/21_July_2008 | < Previous entry]] | + | [[Alberta_NINT/21_July_2008 | < Previous entry]] | [[Alberta_NINT/23_July_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells. | Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells. | ||
| - | Growth visible in K102022 and XL1-B wells. | + | Growth visible in K102022 and XL1-B wells. Put 96-well plate back at 37 C. |
Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and | Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and | ||
plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35. | plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35. | ||
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Latest revision as of 23:01, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells.
Growth visible in K102022 and XL1-B wells. Put 96-well plate back at 37 C.
Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and
plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35.
