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Alberta NINT/25 July 2008
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lab work (SD): | lab work (SD): | ||
Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550 | Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550 | ||
| - | and A420 readings were taken. | + | and A420 readings were taken. Miller units were calculated using the formula |
| - | + | Miller Units = 1000 x [(A420 - 1.75 x A550] / (T x V x 4 x A600) | |
| - | [[ | + | where A420 is detection of yellow |
| + | A550 is detection of cell debris | ||
| + | T = time of reaction in minutes | ||
| + | V = volume of culture used in the assay in mLs | ||
| + | A600 is detection of cell density | ||
| + | [[Media:Alberta_NINT_LacZ_assay_July_24.xls]] | ||
Latest revision as of 23:03, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550
and A420 readings were taken. Miller units were calculated using the formula
Miller Units = 1000 x [(A420 - 1.75 x A550] / (T x V x 4 x A600)
where A420 is detection of yellow
A550 is detection of cell debris
T = time of reaction in minutes
V = volume of culture used in the assay in mLs
A600 is detection of cell density
Media:Alberta_NINT_LacZ_assay_July_24.xls
