Team:Montreal/Notebook

From 2008.igem.org

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Latest revision as of 17:22, 1 August 2008

Home	The Team	The Project	Parts	Notebook	Modeling	Links	Sponsors

Lab Protocols

Key Elements of the Central Dogma of cloning

1. Restriction Digest

2. Making a DNA Agarose Gel

3. Gel extraction

4. DNA quantity assessment

5. Ligation

6. Cell Transformation

7. Seeding method

8. DNA Extraction: Mini-, Midi-, Maxiprep

Lab Progress

@@ Line 1: / Line 1: @@
-{| style="color:#ffffff;background-color:#cd0000;" cellpadding="3" cellspacing="5" border="2" bordercolor="#ffffff" width="75%" align="center"
+{|style="font color="#ffffff"; background-color:#cd0000; cellpadding="3" cellspacing="5" border="2" bordercolor="#cd0000"border-spacing:6px; text-align:center" width="960px"
-!align="center"|[[Team:Montreal|Home]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal|<font color="#ffffff">Home</font>]]
-!align="center"|[[Team:Montreal/Team|The Team]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Team|<font color="#ffffff">The Team</font>]]
-!align="center"|[[Team:Montreal/Project|The Project]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Project|<font color="#ffffff">The Project</font>]]
-!align="center"|[[Team:Montreal/Parts|Parts Submitted to the Registry]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Parts|<font color="#ffffff">Parts</font>]]
-!align="center"|[[Team:Montreal/Modeling|Modeling]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Notebook|<font color="#ffffff">Notebook</font>]]
-!align="center"|[[Team:Montreal/Notebook|Notebook]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Modeling|<font color="#ffffff">Modeling</font>]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Links|<font color="#ffffff">Links</font>]]
+!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Sponsors|<font color="#ffffff">Sponsors</font>]]
 |}
-{| align="center"
-|align="center" |[[Image:Small mcgill igem.jpg]]
+==Lab Protocols==
+{| align="right"
+|align="right" |[[Image:Central_dogma.jpg]]
 |}
-==Lab Progress==
+Key Elements of the Central Dogma of cloning
-'''May 22nd, 2008''': - Prepared TOP10 Competent cells for eventual transformation.
+. [https://2008.igem.org/Team:Montreal/Restriction_digest Restriction Digest]
-- Performed Mini-prep on Reporter+ Cells
-- Performed Digest and Gel on Reporter plasmid extract -- no DNA present, suggest follow-up maxi-prep
+. [https://2008.igem.org/Team:Montreal/DNA_Agarose_Gel Making a DNA Agarose Gel]
+. [https://2008.igem.org/Team:Montreal/Gel_extraction Gel extraction]
+. [https://2008.igem.org/Team:Montreal/Confirmation_quantity DNA quantity assessment]
+. [https://2008.igem.org/Team:Montreal/Ligation Ligation]
+. [https://2008.igem.org/Team:Montreal/Cell_transformation Cell Transformation]
+. [https://2008.igem.org/Team:Montreal/Seeding Seeding method]
+. [https://2008.igem.org/Team:Montreal/DNA_Extraction DNA Extraction: Mini-, Midi-, Maxiprep]
+==Lab Progress==
+<p><center>
+'''[[Team:Montreal/Notebook/May|May 2008]]''' | '''[[Team:Montreal/Notebook/June|June 2008]]''' | '''[[Team:Montreal/Notebook/July|July 2008]]''' | '''[[Team:Montreal/Notebook/August|August 2008]]''' | '''[[Team:Montreal/Notebook/September|September 2008]]''' | '''[[Team:Montreal/Notebook/October|October 2008]]''' | '''[[Team:Montreal/Notebook/November|November 2008]]'''</center><br>