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Team:ESBS-Strasbourg/Concentrations Measurements
From 2008.igem.org
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'''1)by spectrophotometer''' | '''1)by spectrophotometer''' | ||
| - | step1: Prepare your sample | + | step1: Prepare your sample: <br> |
| - | + | 2µL of the solution <br> | |
| + | 400µL Water <br> | ||
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step2: put lambda=260nm <br> | step2: put lambda=260nm <br> | ||
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step3: do the blank with the same water you used for dilution <br> | step3: do the blank with the same water you used for dilution <br> | ||
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step4: Do the conversion absorbance->concentrations <br> | step4: Do the conversion absorbance->concentrations <br> | ||
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=>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL. <br> | =>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL. <br> | ||
| - | '''By nanodrop''' | + | |
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| + | '''2)By nanodrop''' | ||
Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution. <br> | Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution. <br> | ||
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| + | [[Team:ESBS-Strasbourg/Protocols|''Protocols'']] | ||
Latest revision as of 13:09, 29 July 2008
1)by spectrophotometer
step1: Prepare your sample:
2µL of the solution
400µL Water
step2: put lambda=260nm
step3: do the blank with the same water you used for dilution
step4: Do the conversion absorbance->concentrations
1DO = 50µgDNA/mL = 50ng/µL
=>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL.
2)By nanodrop
Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution.
