Team:KULeuven/Project/Reset

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Latest revision as of 14:51, 3 October 2008

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Reset

BioBricks

Components

The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 BBa_I712074]) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 BBa_J23078]). The aiiA gene ([http://partsregistry.org/Part:BBa_C0060 BBa_C0060]), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])

Action

This device was conceived to reset the Timer.

When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.

Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.

@@ Line 1: / Line 1: @@
+{{:Team:KULeuven/Tools/Styling}}
+{{:Team:KULeuven/Tools/Scripting}}
 {{:Team:KULeuven/Tools/Header}}
-<div style="float: right;">[[Image:pictogram_reset.png|120px]]</div>
+[[Image:logo_reset.jpg|120px|right]]
 ==Reset==
-'''figuur'''
+===BioBricks===
+<div style="float:right">[[Image:lactonase.png|330px]]</div>
+[[Image:project_reset.jpg|center]]
-The reset mechanism is made to destroy all HSL ('''link''') at the time, when input re-emerges. Originally, a pulse of lactonase ('''figuur''') was thought to do the thing, but modeling proved that the amount of lactonase was not enough. This simplified the device a lot.
+===Components===
-The aiiA gene ('''BBa_C0060''') codes for lactonase, which breaks down HSL. It is regulated by the output signal from the filter, so that background input signal does not result in timer reset.
+The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 '''BBa_I712074''']) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 '''BBa_J23078''']). The ''aiiA'' gene ([http://partsregistry.org/Part:BBa_C0060 '''BBa_C0060''']), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])
+===Action===
+This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter Timer].
+When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.
+Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.
+{{:Team:KULeuven/Tools/Components}}