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Illinois/29 July 2008
From 2008.igem.org
(Difference between revisions)
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| + | '''LAB WORK''' | ||
| + | |||
'''Antibody/GPCR Fusion''' | '''Antibody/GPCR Fusion''' | ||
*The following were obtained from Courtney Evans to perform DNA extraction from yeast: | *The following were obtained from Courtney Evans to perform DNA extraction from yeast: | ||
**Glass Beads | **Glass Beads | ||
**25:24:1 phenol/chloroform/isoamyl alcohol | **25:24:1 phenol/chloroform/isoamyl alcohol | ||
| + | |||
| + | '''Receptor Tyrosine Kinase method''' | ||
| + | |||
| + | all primers brought to standard concentration, 30mM | ||
| + | |||
| + | 33.3μL H2O added per n mole of primer | ||
Latest revision as of 16:06, 30 July 2008
LAB WORK
Antibody/GPCR Fusion
- The following were obtained from Courtney Evans to perform DNA extraction from yeast:
- Glass Beads
- 25:24:1 phenol/chloroform/isoamyl alcohol
Receptor Tyrosine Kinase method
all primers brought to standard concentration, 30mM
33.3μL H2O added per n mole of primer
