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Team:University of Ottawa/28 July 2008
From 2008.igem.org
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::<li> Control for PCR Reaction was ddH2O instead of DNA template | ::<li> Control for PCR Reaction was ddH2O instead of DNA template | ||
| + | ::<li> 12 PCR Reactions with identical DNA template | ||
| + | '''Matt''' | ||
| + | :'''PCR cleanup''' | ||
| + | ::<li> Performed a PCR cleanup of LiG ptp2/Pssa42 | ||
| + | :'''Transformation''' | ||
| + | ::<li> Transformed Ligated PTP2/pSSA42 into competent cells. | ||
| + | :'''Inoculation''' | ||
| + | ::<li> Inoculated more pSSA42 vector for future digestion/ligations. | ||
Latest revision as of 18:47, 7 August 2008
Today in the Lab
Tammy and Dan
| Sample | Concentration 1 (ng/μL) | V1 (μL) | Concentration 2 (ng/μL) | V2 (μL) | H2O (μL) |
| T123 | 75 | 4 | 5 | 60 | 56 |
- PCR Amplification of T123
| PCR Reaction components | 1X Vol (μl) | 13X Vol (μL) |
| 5X Reaction Buffer | 10 | 130 |
| 10 mM each dNTP | 1 | 13 |
| F Primer (10 pmol/μL) F69 | 2.5 | 32.5 |
| R Primer (10 pmol/μL) F70 | 2.5 | 32.5 |
| DNA Template | 4 | 52 |
| Phusion Polymerase | 0.5 | 6.5 |
| Filtered Sterile ddH2O | 29.5 | 383.5 |
- Control for PCR Reaction was ddH2O instead of DNA template
- 12 PCR Reactions with identical DNA template
Matt
- PCR cleanup
- Performed a PCR cleanup of LiG ptp2/Pssa42
- Transformation
- Transformed Ligated PTP2/pSSA42 into competent cells.
- Inoculation
- Inoculated more pSSA42 vector for future digestion/ligations.
