Team:METU Turkey/Project

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|align="center"|[[Team:METU_Turkey | Team Example 2]]
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:METU_Turkey/Notebook|Notebook]]
!align="center"|[[Team:METU_Turkey/Notebook|Notebook]]
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== The Team ==
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|METU_IGEM  team was established by the 4 undergraduate students one year ago with just the effort of these student. Then 2 members participated. After 2007 competition, thanks to the advises of old four members the recent team was formed. The team composed of 16 undergraduate students majoring in Molecular Biology and Genetics and Biology. We were the first Turkish team which participated in jamboree from Turkey. Hence we also wanted to be the first awarded Turkish team.
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== '''Overall project''' ==
 
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We are trying to modify E. coli in order to carry metals in atomic level. To do this, we try to successively express a metal binding domain to bind metals, a bacteriorhodopsin to seperate
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== The Project ==
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== Project Details==
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'''Light controlled heavy metal carrying ''E. coli'' machine'''
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Heavy metals are  natural components of the Earth's crust. They cannot be degraded or destroyed. To a small extent they enter our bodies via food, drinking water and air. As trace elements, some heavy metals (e.g. copper, selenium, zinc) are essential to maintain the metabolism of the human body. However, at higher concentrations they can lead to poisoning. Heavy metal poisoning could result, for instance, from drinking-water contamination (e.g. lead pipes), high ambient air concentrations near emission sources, or intake via the food chain.
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We designed a ''E.coli'' machine to  control heavy metal concentration in water  by using phototaxis induced tunable carrying system on ''E.coli'' membrane.  In our system, there are two light sources. One of them is for the metal source and another is for the destination to where we want to carry metal atoms. And our machines have phototaxis ability. In addition to this, they have metal binding domain in outer surface and bacteriorhodopsin. When we open light at the metal source, they start to move toward source and catch the metal atoms. Then we open the light at the destination while closing the light at the source. So our machines move toward the destination. After the arrival our machines will be exposed to light with specific wavelength to activate bacteriorhodopsin leading to small local pH changing at the outer surface. This pH changing causes the release of metal atoms from the metal binding domain. Eventually, system starts from the beginning and so on.''
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As a result we will make ''E.coli'' cells act as trucks that carry metal ions on our medium from the metal uptake location to the release locations and also the path are illumintated by lights at specific wavelenghts.''
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<!--- The Mission, Experiments --->
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=== Part 2 ===
 
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== '''Overall project''' ==
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== Project Details==
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 13:41, 4 August 2008

Bilim Agaci.jpg
Home The Team The Project Parts Submitted to the Registry Modeling Notebook


Contents

The Team

METU_IGEM team was established by the 4 undergraduate students one year ago with just the effort of these student. Then 2 members participated. After 2007 competition, thanks to the advises of old four members the recent team was formed. The team composed of 16 undergraduate students majoring in Molecular Biology and Genetics and Biology. We were the first Turkish team which participated in jamboree from Turkey. Hence we also wanted to be the first awarded Turkish team.


The Project

Light controlled heavy metal carrying E. coli machine

Heavy metals are natural components of the Earth's crust. They cannot be degraded or destroyed. To a small extent they enter our bodies via food, drinking water and air. As trace elements, some heavy metals (e.g. copper, selenium, zinc) are essential to maintain the metabolism of the human body. However, at higher concentrations they can lead to poisoning. Heavy metal poisoning could result, for instance, from drinking-water contamination (e.g. lead pipes), high ambient air concentrations near emission sources, or intake via the food chain.

We designed a E.coli machine to control heavy metal concentration in water by using phototaxis induced tunable carrying system on E.coli membrane. In our system, there are two light sources. One of them is for the metal source and another is for the destination to where we want to carry metal atoms. And our machines have phototaxis ability. In addition to this, they have metal binding domain in outer surface and bacteriorhodopsin. When we open light at the metal source, they start to move toward source and catch the metal atoms. Then we open the light at the destination while closing the light at the source. So our machines move toward the destination. After the arrival our machines will be exposed to light with specific wavelength to activate bacteriorhodopsin leading to small local pH changing at the outer surface. This pH changing causes the release of metal atoms from the metal binding domain. Eventually, system starts from the beginning and so on.

As a result we will make E.coli cells act as trucks that carry metal ions on our medium from the metal uptake location to the release locations and also the path are illumintated by lights at specific wavelenghts.



Overall project

Project Details

Retrieved from "http://2008.igem.org/Team:METU_Turkey/Project"