Team:Tokyo Tech/Project

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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Tokyo_Tech|Home]]
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!align="center"|[[Team:Tokyo_Tech/Project|The Project]]
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!align="center"|[[Team:Tokyo_Tech/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Tokyo_Tech/Modeling|Modeling]]
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!align="center"|[[Team:Tokyo_Tech/Japanese|Japanese]]
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!align="center"|[[Team:Tokyo_Tech/Team|The Team]]
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!align="center"|[[Team:Tokyo_Tech/Notebook|Notebook]]
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[[Team:Tokyo_Tech/Mechanism|Mechanism]]
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'''Code name
 
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Rising Sun'''
 
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'''''E.coli UKIYO-E'''''
 
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This project aims to make ''UKIYO-E'' by E.coli.
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== <font size=5>'''Our project''' </font>==
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''UKIYO-E'' is a genre of Japanese woodblock prints.
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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace. 
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<p class=MsoNormal><b><font size=4>We want to make a <i style='mso-bidi-font-style:normal'>Coli Touch!!</font></b></p>
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<td class="a" width="3%">　</td>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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<p class=MsoNormal>We try to construct a bacterial ‘touch panel’ which is colored by the pressure.</p></td>
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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<td class="a" width="3%">　</td>
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|align="center"|[[Team:Tokyo_Tech | Team Example 2]]
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<p class=MsoNormal>We name it <i>Coli touch.</i></p></td>
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<td class="a" width="3%">　</td>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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!align="center"|[[Team:Tokyo_Tech|Home]]
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!align="center"|[[Team:Tokyo_Tech/Team|The Team]]
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          <td class="a" width="3%">　</td>
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!align="center"|[[Team:Tokyo_Tech/Project|The Project]]
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          <td class="a">
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!align="center"|[[Team:Tokyo_Tech/Parts|Parts Submitted to the Registry]]
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<p class=MsoNormal>Until iGEM2007, input style was only heat, small molecule and light. These input
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!align="center"|[[Team:Tokyo_Tech/Modeling|Modeling]]
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style have problems. For example, Heat input occur thermal gradient, small
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!align="center"|[[Team:Tokyo_Tech/Notebook|Notebook]]
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molecule input occur concentration gradient, light input depend on location of a
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|}
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container. So, we thought input style except these. That is “press”. Press input
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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has an advantage that input signal travel uniformly in a container.</span></p></td>
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<td class="a" width="3%">　</td>
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== <font size=5>'''Mechanism'''</font> ==
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== '''Overall project''' ==
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<p class=MsoNormal>We construct genetic circuits that can respond to pressure signal. Lac promoter was known as a pressure inducible promoter. Lac promoter is repressed by LacI protein. The repression is released by adding IPTG (Isopropyl β-D-1-thiogalactopyranoside). Besides IPTG induction, pressure induction is possible. Under high pressure, the affinity of lac protein for lac operon decreases by transition of Lac protein from tetramer to dimer. Through this mechanism, Lac promoter is induced by pressure. </span></p></td>
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Your abstract
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/b/bd/Test.jpg" alt="[サンプルの絵]" width="300">
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== <font size=5>'''Strategy'''</font> ==
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<p class=MsoNormal>We thought two projects, basic project and application project. Basic project consist of two categories. One is construction of pressure responsive genetic (1). The other is creating basic device for a touch display (2). Application project also consist of two categories. One is development of low pressure inducible promoter (3). The other is construction of pressure responsive toggle switch (4). In the future, we want to create the touch display made of E. coli.  </p></td>
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== Project Details==
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/c/cf/Test2.jpg" alt="[サンプルの絵]" width="700">
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<p class=MsoNormal>We used these equipments (show figure) for pressure experiments. </p></td>
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          <td class="a" width="3%">　</td>
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        </tr>
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      </table>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/a/a3/Tech_kaatu.jpg" align="middle" width="500">
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=== <font size=5>'''Construction'''</font> ===
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=== Part 2 ===
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<p class=MsoNormal>We construct these plasmids (show figure). </p></td>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/6/63/Tech_constraction.jpg" align="middle">
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<p class=MsoNormal>Each part is constructed on low-copy plasmid “pSB6”. </p></td>
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=== The Experiments ===
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=== <font size=5>'''Basic device'''</font> ===
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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<p class=MsoNormal>We created pressurizing device for first step of creating touch display. This device has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water. The other (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.                        </p></td>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/9/9d/Tech_youki.jpg" align="middle" width="500" >
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=== Part 3 ===
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=== <font size=5>'''Development of low pressure inducible promoter'''</font> ===
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<body>
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  <div class=Section1 style='layout-grid:18.0pt'>
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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<p class=MsoNormal>In the stage of basic project, very high pressure (30MPa) is required for pressure induction of lac promoter. But lower pressure response is needed for creating touch display. Therefore we develop lower pressure inducible promoter. We performed random mutagenesis to lac promoter and tried to screen promoters with FACS.</p></td>
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          <td class="a" width="3%">　</td>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/3/3e/Tech_dev1.jpg" align="middle" width="500" >
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=== <font size=5>'''Genetic toggle switch to implement rewritable function'''</font> ===
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<p class=MsoNormal>A touch display are required a function of rewritable. Rewritable function consists of writable and erasable one. We adopted a genetic toggle switch that responds to pressure (show figure)</p></td>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<IMG src="https://static.igem.org/mediawiki/2008/5/56/Tech_4toggle1.jpg" align="middle" width="500" >
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== <font size=5>'''Our Team'''</font> ==
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== Results ==
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== <font size=5>'''NoteBook'''</font> ==

Latest revision as of 03:40, 22 October 2008

Home The Project Parts Submitted to the Registry Modeling Japanese The Team Notebook

Mechanism


Contents

Our project

　

We want to make a Coli Touch!!

　
　

We try to construct a bacterial ‘touch panel’ which is colored by the pressure.

 　
　

We name it Coli touch.

 　
　

Until iGEM2007, input style was only heat, small molecule and light. These input style have problems. For example, Heat input occur thermal gradient, small molecule input occur concentration gradient, light input depend on location of a container. So, we thought input style except these. That is “press”. Press input has an advantage that input signal travel uniformly in a container.

 　

Mechanism

　

We construct genetic circuits that can respond to pressure signal. Lac promoter was known as a pressure inducible promoter. Lac promoter is repressed by LacI protein. The repression is released by adding IPTG (Isopropyl β-D-1-thiogalactopyranoside). Besides IPTG induction, pressure induction is possible. Under high pressure, the affinity of lac protein for lac operon decreases by transition of Lac protein from tetramer to dimer. Through this mechanism, Lac promoter is induced by pressure.

　
          [サンプルの絵]


Strategy

　

We thought two projects, basic project and application project. Basic project consist of two categories. One is construction of pressure responsive genetic (1). The other is creating basic device for a touch display (2). Application project also consist of two categories. One is development of low pressure inducible promoter (3). The other is construction of pressure responsive toggle switch (4). In the future, we want to create the touch display made of E. coli.

　
          [サンプルの絵]
　

We used these equipments (show figure) for pressure experiments.

　
          

Construction

　

We construct these plasmids (show figure).

　
          
　

Each part is constructed on low-copy plasmid “pSB6”.

　

Basic device

　

We created pressurizing device for first step of creating touch display. This device has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water. The other (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.

　
          

Development of low pressure inducible promoter

　

In the stage of basic project, very high pressure (30MPa) is required for pressure induction of lac promoter. But lower pressure response is needed for creating touch display. Therefore we develop lower pressure inducible promoter. We performed random mutagenesis to lac promoter and tried to screen promoters with FACS.

 　
          

Genetic toggle switch to implement rewritable function

　

A touch display are required a function of rewritable. Rewritable function consists of writable and erasable one. We adopted a genetic toggle switch that responds to pressure (show figure)

 　
          

Our Team

NoteBook

Retrieved from "http://2008.igem.org/Team:Tokyo_Tech/Project"