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Wisconsin: Lignin Project/10 July 2008
From 2008.igem.org
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| - | Started the following cultures: | + | {|align="justify" style="color:#aada84;background-color:#000;" width="800 px" |
| - | + | |'''Team Sorbitol:'''<br> | |
| - | DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon | + | Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.<br> |
| - | DH5a-pBAD30-srlD: from colony 2 to freeze down for back up | + | Started the following cultures:<br> |
| - | DH5a-pBAD18 - to insert ''srld'' and the operon into | + | DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon<br> |
| - | + | DH5a-pBAD30-srlD: from colony 2 to freeze down for back up<br> | |
| - | MG1655, JW3890, RL257 | + | DH5a-pBAD18 - to insert ''srld'' and the operon into MG1655, JW3890, RL257<br> |
| + | This was done to make chemically competent<br> | ||
| + | '''Team Fungus:'''<br> | ||
| + | Ran out gradient PCR products on 1% agarose gel. No bands.<br> | ||
| + | Ran TAQ gradient PCR again in one more tube due to low amplification results using only 1 uL of cDNA template. | ||
| + | Ran PCR out on gel. Only positive control showed up.<br> | ||
| + | Made LB+Amp+XGal plates.<br> | ||
| + | Attempted transformation again.<br> | ||
| + | |} | ||
Latest revision as of 03:28, 29 October 2008
| Team Sorbitol: Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments. |
