Team:NTU-Singapore/Notebook/26 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: * Darius ** Did a gel run for Lysis terminator. However, unsuccessful gel showed a band between 100bp and 200bp. Correct size of Lysis terminator should be 300bp. *miniprep another 12 tube...)
 
(One intermediate revision not shown)
Line 1: Line 1:
 +
<html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html>
 +
 +
<div id="header">{{User:Greenbear/sandbox/header}}</div>
 +
 +
<div id="maincontent" style="margin-top:130px;">
 +
<html>
 +
<div id="arrow">
 +
<a href="https://2008.igem.org/Team:NTU-Singapore/Notebook">
 +
<img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png
 +
"
 +
    alt="Back to Notebook"
 +
    title="Back to Notebook">
 +
</a>
 +
</div>
 +
</html>
 +
=Saturday 26 July=
 +
<div class="quote" style="margin:20px;">
 +
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
 +
|
* Darius
* Darius
** Did a gel run for Lysis terminator.
** Did a gel run for Lysis terminator.
Line 8: Line 27:
LsrA is successful. band at 140bp. Though abnormality similar to Lysis, a double band is observed. 110bp and 150bp respectively, very close to each other( estimation of values)
LsrA is successful. band at 140bp. Though abnormality similar to Lysis, a double band is observed. 110bp and 150bp respectively, very close to each other( estimation of values)
 +
</div>

Latest revision as of 02:34, 28 October 2008

Saturday 26 July

  • Darius
    • Did a gel run for Lysis terminator.

However, unsuccessful gel showed a band between 100bp and 200bp. Correct size of Lysis terminator should be 300bp.

  • miniprep another 12 tubes of LsrA standard vector innoculated yesterday.
    • digestion of the plasmid extracted.
      • Gel run check

LsrA is successful. band at 140bp. Though abnormality similar to Lysis, a double band is observed. 110bp and 150bp respectively, very close to each other( estimation of values)