Team:BCCS-Bristol/Calendar-Notebook/4 July 2008

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Latest revision as of 09:51, 14 September 2008

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Swimming agar assay

We wanted to test different conditions for the swimming agar assay:

- different agar concentrations

- different incubation temperatures

- different inoculation depths

After an incubation overnight, we measured the diameter of the E. coli MG1655 swarms. Surprisingly, we observed two swarm layers: The lower one has a bigger diameter than the upper.

Agar (%)	Temperature (^o C)	Inoculation Depth	Number	JPG File	Inner Ring (cm)	Outer Ring (cm)
0.3	30	Deep	1	4828	4.6	5.3
0.3	30	Deep	2	4827	4.6	5.3
0.3	30	Deep	3	4829	4.7	5.2
0.3	30	Under Surface	1	4830	4.6	5.2
0.3	30	Under Surface	2	4831	4.6	5.2
0.3	30	Under Surface	3	4832	4.6	5.1
0.3	30	On Surface	1	4833	2.9	3
0.3	30	On Surface	2	4834	3.8	4.4
0.3	30	On Surface	3	4835	4.4	4.9
0.3	37.5	Under Surface	1	4836	7.2	7.6
0.3	37.5	Under Surface	2	4837	7.4	7.7
0.3	37.5	Under Surface	3	4838	7.1	7.4
0.3	25	Under Surface	1	4840	2.8	3.5
0.3	25	Under Surface	2	4839	3	3.6
0.3	25	Under Surface	3	4841	3.1	3.7
0.2	30	Under Surface	1	4842	8.6	8.6
0.2	30	Under Surface	2	4843	8.6	8.6
0.2	30	Under Surface	3	4844	8.6	8.6
0.5	30	Under Surface	1	4847	2.3	2.3
0.5	30	Under Surface	2	4845	1.9	1.9
0.5	30	Under Surface	3	4846	1.5	1.5

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+!align="center"|[[Team:BCCS-Bristol/Team|The Team]]
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 ==Swimming agar assay==
 We wanted to test different conditions for the swimming agar assay: