Team:The University of Alberta/Parts

From 2008.igem.org

(Difference between revisions)
(→Butanol Parts)
 
(32 intermediate revisions not shown)
Line 1: Line 1:
{|  align="center"
{|  align="center"
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta}} https://static.igem.org/mediawiki/2008/9/9a/Home.png]</span><span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Team}} https://static.igem.org/mediawiki/2008/2/2a/Team1.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta}} https://static.igem.org/mediawiki/2008/a/af/HOMEUAB.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Project}} https://static.igem.org/mediawiki/2008/9/92/Project.png]</span><span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Parts}} https://static.igem.org/mediawiki/2008/7/79/Parts.png]</span><span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Modeling}} https://static.igem.org/mediawiki/2008/3/3c/Modeling.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Team}} https://static.igem.org/mediawiki/2008/7/7c/TEAMUA.png]</span>
-
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Notebook}} https://static.igem.org/mediawiki/2008/9/95/Notebook.png]</span>
+
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Project}} https://static.igem.org/mediawiki/2008/7/7e/PROJECTUA.png]</span>
 +
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Parts}} https://static.igem.org/mediawiki/2008/c/c0/PARTSUA.png]</span>
 +
<span class="plainlinks">[{{SERVER}}{{localurl:Team:The_University_of_Alberta/Notebook}} https://static.igem.org/mediawiki/2008/f/f9/NOTEBOOKUA.png]</span>
 +
<br>
<br>
<br>
 +
 +
{| align="center"
 +
[[image:BioBrick.jpg]]
 +
|
 +
 +
==University of Alberta Parts Registry==
 +
 +
<h4>'''All our submitted parts can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=University_of_Alberta in our section of the Parts Registry]!'''</h4> More detailed documentation can be found there for each part.
 +
 +
==Plastic Project==
 +
 +
[[Image:Plastic_Banner.jpg]]
 +
* TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
 +
* PLACIQ + ERE brick - [[Team:The_University_of_Alberta/Parts/Plastic/lacI_ERE_sequence|Sequence]]
 +
* Estrogen Receptor Alpha (Estrogen binding domain)
 +
* Estrogen Receptor Alpha (DNA binding domain)
 +
* BisdA BPA degredation bio brick - [[Team:The_University_of_Alberta/Parts/Plastic/BisDA_sequence|Sequence]]
 +
* BisdB BPA degredation bio brick - [[Team:The_University_of_Alberta/Parts/Plastic/BisDB_sequence|Sequence]]
 +
 +
==Parts Ordered==
 +
PDF Sequences of synthetic genes ordered 20 Jun<br>
 +
*[[Media:UA28-8genes-v2_(2).pdf]]
 +
Genbank files synthetic genes ordered 20 Jun<br>
 +
*[[Media:iGEM08_BisDA.gb]]
 +
*[[Media:iGEM08_BisDB.gb]]
 +
*[[Media:iGEM08_ER01.gb]]
 +
*[[Media:iGEM08_ER02.gb]]
 +
*[[Media:iGEM08_LacIQ_ERE.gb]]
 +
*[[Media:iGEM08_RBS_6XHis.gb]]
 +
*[[Media:iGEM08_TDNA_MCS.gb]]
 +
*[[Media:iGEM08_TetR_RBS_6XHis.gb]]
 +
==Plant Parts==
==Plant Parts==
-
{| align="center"
+
 
[[Image:Plant_Banner.jpg]]
[[Image:Plant_Banner.jpg]]
-
<UL>
+
 
-
<li>Plant CAMV 35S Promoter
+
*Plant CAMV 35S Promoter
-
<li>Hygromycin Resistance Brick
+
*Plant Resistance Brick
-
<li>IGEM Binary Vector
+
**Chloramphenicol was used
 +
*IGEM Binary Vector
 +
**We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
 +
*Blue OX, Purple Russian reporter systems
 +
 
 +
 
 +
==Butanol Parts==
 +
 
 +
<h4> BioBricks Used This Summer</h4>
 +
 
 +
* [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. '''21''')
 +
* [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. '''22''')
 +
* [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. '''23''')
 +
* [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. '''24''')
 +
* [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. '''25''')
 +
* [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
 +
* [http://partsregistry.org/Part:BBa_I0500 I0500]
 +
* [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
 
 +
<h4>Parts Submitted</h4>
 +
 
 +
* [http://partsregistry.org/Part:BBa_S04142 21 + 22]  (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
 +
* [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)
 +
 
 +
<h4>Parts Ordered This Summer</h4>
 +
 
 +
*ETFA  (electron-transfer-flavoprotein, alpha polypeptide)
 +
*ETFB  (electron-transfer-flavoprotein, beta polypeptide)
 +
 
 +
<h4>Parts Assembled This Summer</h4>
 +
<h5>For Protein Expression</h5>
 +
* J61003 + 21
 +
* J61003 + 22
 +
* J61003 + 23
 +
* J61003 + 24
 +
* J61003 + 25
 +
* I0500 + 21
 +
* I0500 + 22
 +
* I0500 + 23
 +
* I0500 + 24
 +
* I0500 + 25
 +
 
 +
<h5>For Butanol Operon</h5>
 +
* 21 + 22  (in plasmid pSB1A3)
 +
* 21 + 22 + 23 (in plasmid pSB1A3)
 +
* 23 + 24 (in plasmid pSB1A3)
 +
* 24 + 25 (in plasmid pSB1A3)
 +
* 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
 +
* ETFA + ETFB (in plasmid Puc57)

Latest revision as of 01:35, 30 October 2008

[http://2008.igem.org/Team:The_University_of_Alberta HOMEUAB.png][http://2008.igem.org/Team:The_University_of_Alberta/Team TEAMUA.png][http://2008.igem.org/Team:The_University_of_Alberta/Project PROJECTUA.png][http://2008.igem.org/Team:The_University_of_Alberta/Parts PARTSUA.png][http://2008.igem.org/Team:The_University_of_Alberta/Notebook NOTEBOOKUA.png]

BioBrick.jpg

Contents

University of Alberta Parts Registry

All our submitted parts can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=University_of_Alberta in our section of the Parts Registry]!

More detailed documentation can be found there for each part.

Plastic Project

Plastic Banner.jpg

  • TetR + LVA tail[http://partsregistry.org/Part:BBa_C0040|]
  • PLACIQ + ERE brick - Sequence
  • Estrogen Receptor Alpha (Estrogen binding domain)
  • Estrogen Receptor Alpha (DNA binding domain)
  • BisdA BPA degredation bio brick - Sequence
  • BisdB BPA degredation bio brick - Sequence

Parts Ordered

PDF Sequences of synthetic genes ordered 20 Jun

Genbank files synthetic genes ordered 20 Jun


Plant Parts

Plant Banner.jpg

  • Plant CAMV 35S Promoter
  • Plant Resistance Brick
    • Chloramphenicol was used
  • IGEM Binary Vector
    • We constructed the IGEM binary vector using P0380 as a starting point. We removed the PUC8 Multiple cloning site from this binary vector and supplemented our own which will allow the vector to accept biobricks.
  • Blue OX, Purple Russian reporter systems


Butanol Parts

BioBricks Used This Summer

  • [http://partsregistry.org/Part:BBa_I725021 RBS + B-hydroxy butyryl CoA dehydrogenase] (AKA. 21)
  • [http://partsregistry.org/Part:BBa_I725022 RBS + Enoyl-CoA hydratase] (AKA. 22)
  • [http://partsregistry.org/Part:BBa_I725023 RBS + Butyryl CoA dehydrogenase] (AKA. 23)
  • [http://partsregistry.org/Part:BBa_I725024 RBS + Butyraldehyde dehydrogenase] (AKA. 24)
  • [http://partsregistry.org/Part:BBa_I725025 RBS + Butanol dehydrogenase] (AKA. 25)
  • [http://partsregistry.org/Part:BBa_J61003 J61003] (without GFP)
  • [http://partsregistry.org/Part:BBa_I0500 I0500]
  • [http://partsregistry.org/Part:pSB1A3 pSB1A3]

Parts Submitted

  • [http://partsregistry.org/Part:BBa_S04142 21 + 22] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04145 21 + 22 + 23] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04143 23 + 24] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04144 24 + 25] (in plasmid pSB1A3)
  • [http://partsregistry.org/Part:BBa_S04147 21 + 22 + 23 + 24 + 25] (in plasmid pSB1A3)

Parts Ordered This Summer

  • ETFA (electron-transfer-flavoprotein, alpha polypeptide)
  • ETFB (electron-transfer-flavoprotein, beta polypeptide)

Parts Assembled This Summer

For Protein Expression
  • J61003 + 21
  • J61003 + 22
  • J61003 + 23
  • J61003 + 24
  • J61003 + 25
  • I0500 + 21
  • I0500 + 22
  • I0500 + 23
  • I0500 + 24
  • I0500 + 25
For Butanol Operon
  • 21 + 22 (in plasmid pSB1A3)
  • 21 + 22 + 23 (in plasmid pSB1A3)
  • 23 + 24 (in plasmid pSB1A3)
  • 24 + 25 (in plasmid pSB1A3)
  • 21 + 22 + 23 + 24 + 25 (in plasmid pSB1A3)
  • ETFA + ETFB (in plasmid Puc57)
Retrieved from "http://2008.igem.org/Team:The_University_of_Alberta/Parts"