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Alberta NINT/30 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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Transformed XL1-B cells with the ligation product. | Transformed XL1-B cells with the ligation product. | ||
| - | + | lab work (JD): | |
| - | + | Ligation of K102011 with TAIn_2, TAIn_3, TAIn_6 and TAIn_7 | |
| + | Transformed XL1-B cells with the ligations | ||
| + | Plated on LB+kan35 plates | ||
| + | Incubated at 37C overnight | ||
Latest revision as of 23:05, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Extracted DNA from gel using QIAquick gel extraction protocol.
Quantified DNA and ligated K102031 /E+P and K102032 /E+P together.
Transformed XL1-B cells with the ligation product.
lab work (JD):
Ligation of K102011 with TAIn_2, TAIn_3, TAIn_6 and TAIn_7
Transformed XL1-B cells with the ligations
Plated on LB+kan35 plates
Incubated at 37C overnight
